Altintas R, Ediz C, Celik H, Camtosun A, Tasdemir C, Tanbek K, Tekin S, Colak C, Alan C
Department of Urology, Inonu University School of Medicine, Malatya, Turkey.
Department of Physiology, Inonu University School of Pharmacy, Malatya, Turkey.
Andrology. 2016 May;4(3):442-6. doi: 10.1111/andr.12172. Epub 2016 Mar 5.
The aim of this prospective controlled study was to investigate the levels of reactive oxygen species (ROS), including asymmetric dimethylarginine (ADMA), oxidative stress index (OSI) and total oxidant capacity (TOC), and antioxidants with total antioxidant capacity (TAC) in peripheral and internal spermatic veins blood, the relationship of these factors with sperm parameters in the infertile varicocoele patients, and the amelioration effect of varicocoelectomy on these outcomes. Thirty-one primary infertile varicocoele patients and 31 fertile control patients evaluated for determining the levels of ADMA, TOC, OSI, superoxide dismutase (SOD), glutathione (GSH), TAC, and semen analysis. The patients' preoperative SOD, GSH and TAC levels, which were significantly lower than the controls, significantly increased postoperatively. Although SOD and GSH were significantly higher in spermatic vein compared to median cubital vein, TAC was significantly higher in median cubital vein. ADMA, TOC and OSI were significantly higher in the patient group. TOC and OSI were significantly higher in spermatic vein compared to median cubital vein. Postoperative TOC, OSI and ADMA reduced to the control levels. Total antioxidant capacity in the peripheral circulation and oxidative stress index in the internal spermatic vein could give an idea about the possible improvement in sperm count acquired by varicocoelectomy.
这项前瞻性对照研究的目的是调查精索外周静脉血和精索内静脉血中活性氧物质（ROS）的水平，包括不对称二甲基精氨酸（ADMA）、氧化应激指数（OSI）和总氧化剂能力（TOC），以及具有总抗氧化能力（TAC）的抗氧化剂，研究这些因素与不育精索静脉曲张患者精子参数的关系，以及精索静脉结扎术对这些结果的改善作用。对31例原发性不育精索静脉曲张患者和31例生育能力正常的对照患者进行评估，以测定ADMA、TOC、OSI、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、TAC水平，并进行精液分析。患者术前的SOD、GSH和TAC水平显著低于对照组，术后显著升高。虽然精索静脉中的SOD和GSH显著高于肘正中静脉，但肘正中静脉中的TAC显著更高。患者组的ADMA、TOC和OSI显著更高。精索静脉中的TOC和OSI显著高于肘正中静脉。术后TOC、OSI和ADMA降至对照水平。外周循环中的总抗氧化能力和精索内静脉中的氧化应激指数可以反映精索静脉结扎术可能使精子数量得到改善的情况。
