Vestergaard Bente
Department of Drug Design and Pharmacology, University of Copenhagen, Jagtvej 162, 2100 Copenhagen, Denmark.
Arch Biochem Biophys. 2016 Jul 15;602:69-79. doi: 10.1016/j.abb.2016.02.029. Epub 2016 Mar 2.
Solution small angle X-ray scattering from biological macromolecules (BioSAXS) plays an increasingly important role in biostructural research. The analysis of complex protein mixtures, dynamic equilibriums, intrinsic disorder and evolving structural processes is facilitated by SAXS data, either in stand-alone applications, or with SAXS taking a prominent role in hybrid biostructural analysis. This is not the least due to the significant advances in both hardware and software that have taken place in particular at the large-scale facilities. Here, recent developments and the future potential of BioSAXS are reviewed, exemplified by numerous examples of elegant applications to challenging systems.
生物大分子的溶液小角X射线散射（BioSAXS）在生物结构研究中发挥着越来越重要的作用。无论是在独立应用中，还是在混合生物结构分析中SAXS发挥重要作用时，SAXS数据都有助于对复杂蛋白质混合物、动态平衡、内在无序和不断演变的结构过程进行分析。这尤其得益于特别是在大型设施中硬件和软件方面取得的重大进展。本文综述了BioSAXS的最新进展及其未来潜力，并通过众多应用于具有挑战性系统的精彩实例加以说明。
