Allione Alessandra, Di Gaetano Cornelia, Dani Nadia, Barberio Davide, Sieri Sabina, Krogh Vittorio, Matullo Giuseppe
Human Genetics Foundation - HuGeF, Via Nizza 52, 10126 Turin, Italy; Department of Medical Sciences, University of Turin, Via Santena 19, 10100 Turin, Italy.
Human Genetics Foundation - HuGeF, Via Nizza 52, 10126 Turin, Italy; Department of Medical Sciences, University of Turin, Via Santena 19, 10100 Turin, Italy.
Cytokine. 2016 Apr;80:43-7. doi: 10.1016/j.cyto.2016.03.001. Epub 2016 Mar 3.
Obesity is an important health problem worldwide. Adipose tissue acts as an endocrine organ that secretes various bioactive substances, called adipokines, including pro-inflammatory biomarkers such as TNF-α, IL-6, leptin and C-reactive protein (CRP) and anti-inflammatory molecules such as adiponectin. The deregulated production of adipokines in obesity is linked to the pathogenesis of various disease processes and monitoring their variation is critical to understand metabolic diseases. The aim of this study was to determine the plasma concentration of adipokines in healthy subjects by multiplexed measurements and the effect of anticoagulants on their levels. Plasma samples from 10 healthy donors were collected in two different anticoagulants (sodium citrate or heparin). All markers, excluding TNF-α, showed significantly higher concentrations in heparinized compared to citrate plasma. However, levels of adipokines in different plasma samples were highly correlated for most of these markers. We reported that different anticoagulants used in the preparation of the plasma samples affected the measurements of some adipokines. The importance of the present results in epidemiology is relevant when comparing different studies in which blood samples were collected with different anticoagulants.
肥胖是一个全球性的重要健康问题。脂肪组织作为一个内分泌器官，分泌各种生物活性物质，即脂肪因子，包括促炎生物标志物如肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、瘦素和C反应蛋白（CRP）以及抗炎分子如脂联素。肥胖状态下脂肪因子的产生失调与各种疾病进程的发病机制相关，监测它们的变化对于理解代谢性疾病至关重要。本研究的目的是通过多重检测确定健康受试者血浆中脂肪因子的浓度以及抗凝剂对其水平的影响。从10名健康供体采集的血浆样本分别置于两种不同的抗凝剂（柠檬酸钠或肝素）中。除TNF-α外，所有标志物在肝素化血浆中的浓度均显著高于柠檬酸盐血浆。然而，对于大多数这些标志物而言，不同血浆样本中脂肪因子的水平高度相关。我们报告称，制备血浆样本时使用的不同抗凝剂会影响某些脂肪因子的检测。当比较使用不同抗凝剂采集血样的不同研究时，本研究结果在流行病学中的重要性就显现出来了。
