Takano H, Takahashi T, Nakata A, Nogami S, Yusa K, Kuwajima S, Yamazaki M, Fukuda M
Department of Dentistry and Oral Surgery, Akita University Graduate School of Medicine, Akita, Japan.
Division of Maxillofacial Surgery, Department of Oral Medicine and Surgery, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, Japan.
J Oral Rehabil. 2016 May;43(5):333-9. doi: 10.1111/joor.12388. Epub 2016 Mar 6.
The aim of this study was to investigate the bone resorption effect of the mediators delivered in joint cavity of patients with mandibular condyle fractures by detecting osteoclast markers using cellular biochemistry methods, and by analysing bone resorption activities via inducing osteoclast differentiation of the infiltrated cells from arthrocentesis. Sixteen joints in 10 patients with mandibular condyle fractures were evaluated. The control group consisted of synovial fluid (SF) samples from seven joints of four volunteers who had no clinical signs or symptoms involving the temporomandibular joint (TMJ) or disc displacement. We collected SF cells from all patients during therapeutic arthrocentesis. The infiltrating cells from TMJ SF were cultured, differentiated into tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells and examined bone resorption activities. We also investigated factors related to osteoclast induction of SF, using ELISA procedures. Osteoclast-like cells were induced from the SF cells obtained from all patients with condylar fractures. These multinucleated giant cells were positive for TRAP and actin, and had the ability to absorb dentin slices. The levels of macrophage colony-stimulating factor (M-CSF), prostaglandin E2 (PGE2), soluble form of receptor activator of nuclear factor kappa-B ligand (sRANKL) and osteoprotegerin (OPG), in SF samples from the patients, were significantly higher than in the controls. These findings indicate that bone resorption activities in SF from patients with mandibular condyle fractures were upregulated and may participate in the pathogenesis and wound healing.
本研究旨在通过细胞生化方法检测破骨细胞标志物，并通过诱导关节穿刺浸润细胞的破骨细胞分化来分析骨吸收活性，以研究在下颌髁突骨折患者关节腔中递送的介质的骨吸收作用。对10例下颌髁突骨折患者的16个关节进行了评估。对照组由4名无颞下颌关节（TMJ）或盘移位临床体征或症状的志愿者的7个关节的滑液（SF）样本组成。在治疗性关节穿刺过程中，我们收集了所有患者的SF细胞。培养来自TMJ SF的浸润细胞，将其分化为抗酒石酸酸性磷酸酶（TRAP）阳性的破骨细胞样细胞，并检测骨吸收活性。我们还使用ELISA程序研究了与SF破骨细胞诱导相关的因素。从所有髁突骨折患者获得的SF细胞中诱导出破骨细胞样细胞。这些多核巨细胞TRAP和肌动蛋白呈阳性，并且具有吸收牙本质切片的能力。患者SF样本中巨噬细胞集落刺激因子（M-CSF）、前列腺素E2（PGE2）、核因子κB受体活化因子配体可溶性形式（sRANKL）和骨保护素（OPG）的水平显著高于对照组。这些发现表明，下颌髁突骨折患者SF中的骨吸收活性上调，可能参与发病机制和伤口愈合。
