刺激唾液和非刺激唾液作为评估可替宁浓度的生物材料。

Stimulated and non-stimulated saliva as biological material in the assessment of cotinine concentration.

作者信息

Bachaneki Teresa, Nakonieczna-Rudnicka Marta, Piekarczyk Wanda

出版信息

Przegl Lek. 2015;72(10):493-5.

Abstract

INTRODUCTION

A biomarker of the exposure to the tobacco smoke is cotinine - a nicotine metabolite with a half-life about 16 hours. Analysis of cotinine concentration in biological material allows objective assessment of the cigarette smoking status and environmental exposure to the tobacco smoke.

AIM OF THE STUDY

The aim of the study was evaluation of the influence of method of saliva collection: stimulated or non-stimulated one by chewing a paraffin cube on cotinine concentration in the saliva as well as the analysis of the obtained cotinine values depending on the status of cigarette smoking.

MATERIAL AND METHODS

Survey with the use of authorial survey questionnaire and biochemical survey of saliva were conducted among 115 people aged 20-50 who reported for treatment at the Department of Conservative Dentistry with Endodontics of the Medical University of Lublin. In the examined group 66 people declared cigarette smoking, 49 reported they had never smoked cigarettes and had never tried to smoke. Cotinine concentration in the saliva was assayed with the use of Cotinine test (Calbiotech). Obtained study results were submitted to statistical analysis using Ch2 test. Statistically essential test values were those of p<0.05. While investigating the influence of age on the examined parameters Spearman correlation coefficient and the test of its relevance were employed.

RESULTS

Mean cotinine concentra- tion in non-stimulated saliva in smokers was 310.36 ng/ml, in stimulated saliva 305.61 ng/ml. No statistically essential differences were stated between stimulated and non-stimulated saliva (Z=0.36, p>0.05). Cotinine concentration in non-stimulated and in stimulated saliva was increasing with age of the investigated.

CONCLUSIONS

Cotinine concentration in both in stimulated and non-stimulated saliva obtains similar values. Cotinine concentration both in stimulated and non-stimulated saliva increases with age of the investigated.

摘要

引言

可替宁是烟草烟雾暴露的生物标志物——一种半衰期约为16小时的尼古丁代谢物。分析生物材料中的可替宁浓度有助于客观评估吸烟状况和环境烟草烟雾暴露情况。

研究目的

本研究旨在评估唾液采集方法（通过咀嚼石蜡块刺激或未刺激）对唾液中可替宁浓度的影响，以及根据吸烟状况分析所获得的可替宁值。

材料与方法

对115名年龄在20至50岁之间、到卢布林医科大学保守牙科与牙髓病科就诊的患者进行了使用自编调查问卷的调查和唾液生化检测。在被检查的群体中，66人宣称吸烟，49人报告他们从未吸烟且从未尝试吸烟。使用可替宁检测试剂盒（Calbiotech）测定唾液中的可替宁浓度。所获得的研究结果采用卡方检验进行统计分析。具有统计学意义的检验值为p<0.05。在研究年龄对所检查参数的影响时，采用了Spearman相关系数及其相关性检验。