狼疮性肾炎患者中白细胞介素-10（IL-10）阳性调节性B细胞（Bregs）减少。
Decreased IL-10(+) regulatory B cells (Bregs) in lupus nephritis patients.
作者信息
Heinemann K, Wilde B, Hoerning A, Tebbe B, Kribben A, Witzke O, Dolff S
机构信息
a Department of Nephrology , University Hospital Essen, University Duisburg-Essen , Essen , Germany.
b Department of Paediatrics and Adolescent Medicine , University Hospital Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg , Erlangen , Germany.
出版信息
Scand J Rheumatol. 2016 Jul;45(4):312-6. doi: 10.3109/03009742.2015.1126346. Epub 2016 Mar 7.
OBJECTIVES
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by B cell-dependent autoantibody production. Recently, a new B-cell subset was discovered that has a regulatory capacity. The aim of this study was to analyse regulatory B cells (Bregs) in SLE patients.
METHOD
Peripheral mononuclear blood cells (PBMCs) of 34 SLE patients fulfilling the American College of Rheumatology (ACR) criteria for SLE and 21 healthy controls (HC) were included. PBMCs were stained for CD19, CD24, and CD38 and analysed by flow cytometry. In vitro stimulated PBMCs with CpG and restimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin were investigated for IL-10(+) Bregs .
RESULTS
The percentages of circulating CD19(+)CD24(hi)CD38(hi) cells in HC were not different those in from SLE patients. The percentages of IL-10(+) Bregs were significantly decreased in SLE patients, in particular those with lupus nephritis (LN), compared to HC. The proportion was independent of disease activity.
CONCLUSIONS
This is the first study to demonstrate a decrease in IL-10-producing B cells in LN patients compared to HC, reflecting an impaired regulatory function.
目的
系统性红斑狼疮（SLE）是一种自身免疫性疾病，其特征为B细胞依赖性自身抗体产生。最近，发现了一种具有调节能力的新B细胞亚群。本研究的目的是分析SLE患者中的调节性B细胞（Bregs）。
方法
纳入34例符合美国风湿病学会（ACR）SLE标准的SLE患者和21例健康对照（HC）的外周血单个核细胞（PBMC）。对PBMC进行CD19、CD24和CD38染色，并通过流式细胞术进行分析。用CpG体外刺激PBMC，并用佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）和离子霉素再次刺激，研究IL - 10(+) Bregs。
结果
HC中循环CD19(+)CD24(hi)CD38(hi)细胞的百分比与SLE患者无差异。与HC相比，SLE患者，尤其是狼疮性肾炎（LN）患者中IL - 10(+) Bregs的百分比显著降低。该比例与疾病活动度无关。
结论
这是第一项证明与HC相比LN患者中产生IL - 10的B细胞减少的研究，反映了调节功能受损。
Zotero 插件