Bennett Kaila M, Parnell Erinn A, Sanscartier Candice, Parks Sophia, Chen Gang, Nair Meera G, Lo David D
Bioengineering Interdepartmental Graduate Program, School of Medicine, University of California, Riverside, Riverside, California; Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California.
Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, California.
Am J Pathol. 2016 May;186(5):1166-79. doi: 10.1016/j.ajpath.2015.12.015. Epub 2016 Mar 3.
Intestinal M (microfold) cells are specialized epithelial cells overlying lymphoid tissues in the small intestine. Unlike common enterocytes, M cells lack an organized apical brush border, and are able to transcytose microparticles across the mucosal barrier to underlying antigen-presenting cells. We found that in both the dextran sodium sulfate and Citrobacter rodentium models of colitis, significantly increased numbers of Peyer's patch (PP) phenotype M cells were induced at the peak of inflammation in colonic epithelium, often accompanied by loosely organized lamina propria infiltrates. PP type M cells are thought to be dependent on cytokines, including tumor necrosis factor (TNF)-α and receptor activator of nuclear factor kappa-B ligand; these cytokines were also found to be induced in the inflamed tissues. The induction of M cells was abrogated by anti-TNF-α blockade, suggesting that anti-TNF-α therapies may have similar effects in clinical settings, although the functional consequences are not clear. Our results suggest that inflammatory cytokine-induced PP type M cells may be a useful correlate of chronic intestinal inflammation.
肠道M（微褶）细胞是覆盖在小肠淋巴组织上的特化上皮细胞。与普通肠上皮细胞不同，M细胞缺乏有组织的顶端刷状缘，并且能够跨细胞转运微粒穿过黏膜屏障至其下方的抗原呈递细胞。我们发现在葡聚糖硫酸钠和鼠柠檬酸杆菌结肠炎模型中，结肠上皮炎症高峰期诱导出的派尔集合淋巴结（PP）表型M细胞数量显著增加，常伴有固有层浸润组织松散。PP型M细胞被认为依赖于细胞因子，包括肿瘤坏死因子（TNF）-α和核因子κB受体激活剂配体；这些细胞因子在炎症组织中也被发现有诱导作用。抗TNF-α阻断可消除M细胞的诱导，这表明抗TNF-α疗法在临床环境中可能有类似作用，尽管其功能后果尚不清楚。我们的结果表明，炎症细胞因子诱导的PP型M细胞可能是慢性肠道炎症的一个有用相关指标。
