College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Food Chem. 2016 Jul 15;203:498-504. doi: 10.1016/j.foodchem.2016.02.087. Epub 2016 Feb 15.
Alcohol acetyltransferase (AATFase) extensively catalyzes the reactions of alcohols to acetic esters in microorganisms and plants. In this work, a novel method has been proposed to quantify the activity of AATFase using a SnO2-based sensor of electronic nose, which was determined on the basis of its higher sensitivity to the reducing alcohol than the oxidizing ester. The maximum value of the first-derivative of the signals from the SnO2-based sensor was therein found to be an eigenvalue of isoamyl alcohol concentration. Quadratic polynomial regression perfectly fitted the correlation between the eigenvalue and the isoamyl alcohol concentration. The method was used to determine the AATFase activity in this type of reaction by calculating the conversion rate of isoamyl alcohol. The proposed method has been successfully applied to determine the AATFase activity of a cider yeast strain. Compared with GC-MS, the method shows promises with ideal recovery and low cost.
醇乙酰基转移酶（AATFase）广泛催化微生物和植物中醇类向乙酸酯的反应。在这项工作中，提出了一种新的方法，使用基于 SnO2 的电子鼻传感器来定量测定 AATFase 的活性，该方法基于其对还原醇的灵敏度高于氧化酯。发现基于 SnO2 的传感器信号的一阶导数的最大值是异戊醇浓度的特征值。二次多项式回归完美拟合了特征值与异戊醇浓度之间的相关性。该方法通过计算异戊醇的转化率，用于通过此类反应来确定 AATFase 活性。该方法已成功应用于 cider 酵母菌株的 AATFase 活性的测定。与 GC-MS 相比，该方法具有理想的回收率和低成本的优势。
