Rahyussalim Ahmad Jabir, Kurniawati Tri, Siregar Nurjati Chairani, Syahrurachman Agus, Dilogo Ismail Hadisubroto, Iskandriati Diah, Fitri Arni Diana
Department of Orthopaedic and Traumatology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
Stem Cell and Tissue Engineering Cluster, MERC Faculty of Medicine University of Indonesia, Jakarta, Indonesia.
Asian Spine J. 2016 Feb;10(1):1-5. doi: 10.4184/asj.2016.10.1.1. Epub 2016 Feb 16.
Preliminary experimental study using a rabbit spondylitis model.
To observe the ossification in a micro-environment containing live Mycobacterium tuberculosis transplanted with bone marrow stromal cells (BMSCs) in rabbits.
BMSCs differentiate to osteoblasts and then osteocytes during ossification. Mycobacterium tuberculosis does not affect BMSC growth in vitro.
Six rabbits were divided into two groups of three rabbits. One group was positive for spondylitis tuberculosis by culture, polymerase chain reaction (PCR), and histopathologically. The other group was positive by PCR and histopathologically. Both groups were treated using BMSC transplantation and anti-tuberculosis drugs. After 6 weeks, ossification was evaluated by enumerating the number of osteoblasts, osteocytes, and lesion level of calcium.
Mean number of osteoblasts was 207.00±31.00 in the first group and 220.33±73.46 in the second group. Mean number of intra-lesions osteocytes was in the first and second group was 18.33±30.04 and 31.00±26.87, respectively. Mean calcium level in the first group and second group was 2.94%±0.89% and 2.51%±0.13%, respectively. Total ossification score in the first and second group was 31.00 and 25.67, respectively.
Mycobacterium tuberculosis provides support for new bone formation by stimulating intra-lesion calcium metabolism. The microscopic environment containing live Mycobacterium tuberculosis enhances ossification.
使用兔脊柱炎模型的初步实验研究。
观察在移植了骨髓基质细胞（BMSCs）的含有活结核分枝杆菌的微环境中兔的骨化情况。
骨髓基质细胞在骨化过程中分化为成骨细胞，然后再分化为骨细胞。结核分枝杆菌不影响骨髓基质细胞在体外的生长。
将6只兔子分为两组，每组3只。一组通过培养、聚合酶链反应（PCR）和组织病理学检查确诊为脊柱结核阳性。另一组通过PCR和组织病理学检查为阳性。两组均采用骨髓基质细胞移植和抗结核药物治疗。6周后，通过计数成骨细胞、骨细胞数量和钙的病变水平评估骨化情况。
第一组成骨细胞的平均数量为207.00±31.00，第二组为220.33±73.46。第一组和第二组病变内骨细胞的平均数量分别为18.33±30.04和31.00±26.87。第一组和第二组钙的平均水平分别为2.94%±0.89%和2.51%±0.13%。第一组和第二组的总骨化评分分别为31.00和25.67。
结核分枝杆菌通过刺激病变内钙代谢为新骨形成提供支持。含有活结核分枝杆菌的微观环境可增强骨化。
