基于转录组的泽米铁科铁树属物种的微卫星标记
Transcriptome-derived microsatellite markers for Dioon (Zamiaceae) cycad species.
作者信息
Prado Alberto, Cervantes-Díaz Fret, Perez-Zavala Francisco G, González-Astorga Jorge, Bede Jacqueline C, Cibrián-Jaramillo Angélica
机构信息
Department of Plant Science, McGill University, 21,111 Lakeshore, Ste-Anne-de-Bellevue, Québec, Canada.
Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Unidad de Genómica Avanzada, Cinvestav, Km 9.6 Libramiento Norte Carr. Irapuato-León, 36821 Irapuato, Mexico.
出版信息
Appl Plant Sci. 2016 Feb 12;4(2). doi: 10.3732/apps.1500087. eCollection 2016 Feb.
PREMISE OF THE STUDY
Dioon (Zamiaceae) is an endangered North American cycad genus of evolutionary and ornamental value. We designed and validated a set of microsatellite markers from D. edule that can be used for population-level and conservation studies, and that transferred successfully to D. angustifolium, D. spinulosum, and D. holmgrenii.
METHODS AND RESULTS
We tested 50 primers from 80 microsatellite candidate loci in the OneKP D. edule transcriptome. Genotypes from 21 loci in 20 D. edule individuals revealed up to 14 alleles per locus and observed heterozygosity from 0.15 to 0.92; one locus was monomorphic. Seven of those 21 loci were polymorphic in D. angustifolium, D. spinulosum, and D. holmgrenii, with up to seven alleles, and an observed heterozygosity up to 0.89.
CONCLUSIONS
The transcriptome-derived microsatellites generated here will serve as tools to advance population genetic studies and inform conservation strategies of Dioon, including the identification and origin of illegal plants in the cycad trade.
研究前提
铁树属（泽米科）是一种濒危的北美苏铁属植物，具有进化和观赏价值。我们设计并验证了一组来自矮型铁树的微卫星标记，可用于种群水平和保护研究，并成功转移至狭叶铁树、多刺铁树和霍氏铁树。
方法与结果
我们在1000种植物转录组计划的矮型铁树转录组中测试了80个微卫星候选位点中的50对引物。20株矮型铁树个体中21个位点的基因型显示每个位点多达14个等位基因，观察到的杂合度为0.15至0.92；一个位点为单态。这21个位点中的7个在狭叶铁树、多刺铁树和霍氏铁树中具有多态性，多达7个等位基因，观察到的杂合度高达0.89。
结论
此处产生的转录组衍生微卫星将作为推进铁树属种群遗传学研究的工具，并为铁树属的保护策略提供信息，包括苏铁贸易中非法植物的识别和来源。
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