来自肠致病性大肠杆菌菌株的pR351质粒的遗传和分子特征分析

Genetic and molecular characterization of pR351 plasmid from enteropathogenic Escherichia coli strain.

作者信息

Czarny A, Lagowska-Złotorzycka M, Mulczyk M

机构信息

Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.

出版信息

Arch Immunol Ther Exp (Warsz). 1989;37(1-2):191-9.

PMID:2695005

Abstract

A pR351 plasmid (Tc Ap Cb) conferring drug resistance of naturally occurring E. coli strain was examined. Conjugation and transduction experiments have indicated that this plasmid is R plasmid aggregate consisting of three independent plasmids: a) conjugative plasmid pR351 A (SuTc) fi- (F) belonging to incompatibility group L, b) conjugative plasmid pR351 B (SuApCb) fi- (F) belonging to incompatibility O, and c) non-conjugative plasmid pR351 C (ApCb). The existance of these plasmids in pR351 plasmid aggregate was confirmed by the agarose gel analysis of plasmid DNA isolated from the lysates of E. coli J53 transconjugants carrying pR351 A or pR351 B plasmids and from E. coli K12 C600 transductant carrying pR351 C plasmid. Molecular mass of these plasmids was found to be 55.60 and 3 Mdal respectively. The non-conjugative pR351 C plasmid could be mobilized by Col B and F factors. Our findings have indicated that two fi- (F) R plasmids can stably coexist in R plasmid aggregate.

摘要

对赋予天然存在的大肠杆菌菌株耐药性的pR351质粒（Tc Ap Cb）进行了检测。接合和转导实验表明，该质粒是由三个独立质粒组成的R质粒聚集体：a）属于不相容群L的接合性质粒pR351 A（SuTc）fi-（F），b）属于不相容群O的接合性质粒pR351 B（SuApCb）fi-（F），以及c）非接合性质粒pR351 C（ApCb）。通过对从携带pR351 A或pR351 B质粒的大肠杆菌J53转接合子裂解物以及携带pR351 C质粒的大肠杆菌K12 C600转导子中分离的质粒DNA进行琼脂糖凝胶分析，证实了这些质粒在pR351质粒聚集体中的存在。发现这些质粒的分子量分别为55.60和3 Mdal。非接合性质粒pR351 C可被Col B和F因子动员。我们的研究结果表明，两个fi-（F）R质粒可以在R质粒聚集体中稳定共存。