Rybalchenko O V, Stepanova O M, Orlova O G, Astafiev A M, Kudryavtsev A A, Kapustina V V
Zh Mikrobiol Epidemiol Immunobiol. 2015 Nov-Dec(6):17-23.
Detection of bactericidal effect of pulse-periodic corona discharge (PPCD) on cells and biofilms of Escherichia coli M17.
A gas-discharge device was created based on PPCD in air with power supply parameters: amplitude values of voltage of 30 - 60 kV, pulse repetition rate of 250 - 400 kHz. Ultrastructure changes in cells and biofilms of E. coli M17, affected by PPCD, generated in air, were studied by typical methods of transmission electron microscopy.
Disturbances of integrity of surface and abyssal structures of biofilms, as well as changes of morphological properties of E. coli M17 cells, characteristic for sub-lethal heat impact, were detected. Destructive changes of bacterial cells were developed by formation of focal disturbance of cytoplasmic membrane, extension of periplasmic space, formation of globular structures, characteristic for heat effect, and destruction of cytoplasm.
Bactericidal effect of PPCD on E. coli M17 cells as part of biofilms was shown. Destructive morphological changes in cells and biofilms of E. coli M17 after the effect of PPCD were detected for the first time on electron-microscopic level.
检测脉冲周期电晕放电（PPCD）对大肠杆菌M17细胞及生物膜的杀菌效果。
基于空气中的PPCD创建了一种气体放电装置，其电源参数为：电压幅值30 - 60 kV，脉冲重复频率250 - 400 kHz。采用透射电子显微镜的典型方法，研究了空气中产生的PPCD对大肠杆菌M17细胞及生物膜超微结构的影响。
检测到生物膜表面和深层结构完整性受到干扰，以及大肠杆菌M17细胞形态特性发生变化，这些变化是亚致死热影响的特征。细菌细胞的破坏性变化表现为细胞质膜形成局灶性干扰、周质空间扩大、形成热效应特征性的球状结构以及细胞质破坏。
显示了PPCD对作为生物膜一部分的大肠杆菌M17细胞的杀菌效果。首次在电子显微镜水平上检测到PPCD作用后大肠杆菌M17细胞及生物膜的破坏性形态变化。
