[从罗斯托夫地区临床样本和水生环境中分离出的沙门氏菌的生物学特性]
[BIOLOGICAL PROPERTIES OF SALMONELLA, ISOLATED FROM CLINICAL MATERIAL AND AQUATIC ENVIRONMENT IN ROSTOV REGION].
作者信息
Panasovets O P, Usatkin A V, Shmailenko O A
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 2015 Nov-Dec(6):90-3.
AIM
Study biological properties of salmonella, isolated from clinical materials and water of Don river.
MATERIALS AND METHODS
Salmonella strains of various serovars were used in the study. Biochemical characteristics were studied by generally accepted methods, antigenic properties were evaluated in agglutination reactions, virulence was determined by Dlm for laboratory animals, antibiotics sensitivity was verified by disc-diffusion method.
RESULTS
The presence of pathogenicity factors in isolated strains was shown: hemolytic activity--in 64 and 36.8% of cases, DNAse activity--in 28 and 26%, respectively in clinical and wild strains. Microorganism dose, resulting in death of all the animals (LD100) did not depend on serovar of salmonella and varied from 10(3) to 10(10) PFU/ml.
CONCLUSION
Clinical strains were established to possess higher virulence and resistance to antibiotics compared with strains isolated from the aquatic envionment.
目的
研究从顿河临床样本和河水分离出的沙门氏菌的生物学特性。
材料与方法
研究中使用了各种血清型的沙门氏菌菌株。通过普遍接受的方法研究生化特性，在凝集反应中评估抗原特性，通过对实验动物进行半数致死量测定来确定毒力，采用纸片扩散法验证抗生素敏感性。
结果
在分离出的菌株中显示出致病因子的存在：溶血活性——临床菌株和野生菌株中分别为64%和36.8%，脱氧核糖核酸酶活性——分别为28%和26%。导致所有动物死亡的微生物剂量（LD100）不取决于沙门氏菌的血清型，范围从10³到10¹⁰ PFU/ml。
结论
已证实临床菌株与从水生环境分离出的菌株相比具有更高的毒力和抗生素抗性。
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