提高 NADPH 产量以增强集胞藻 PCC 6803 的生物质和乙醇产量。
Enhancing biomass and ethanol production by increasing NADPH production in Synechocystis sp. PCC 6803.
机构信息
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea.
Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea; School of Environmental Science and Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea; Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea.
出版信息
Bioresour Technol. 2016 Aug;213:54-57. doi: 10.1016/j.biortech.2016.02.056. Epub 2016 Mar 3.
This study demonstrates that increased NADPH production can improve biomass and ethanol production in cyanobacteria. We over-expressed the endogenous zwf gene, which encodes glucose-6-phosphate dehydrogenase of pentose phosphate pathway, in the model cyanobacterium Synechocystis sp. PCC 6803. zwf over-expression resulted in increased NADPH production, and promoted biomass production compared to the wild type in both autotrophic and mixotrophic conditions. Ethanol production pathway including NADPH-dependent alcohol dehydrogenase was also integrated with and without zwf over-expression. Excessive NADPH production by zwf over-expression could improve both biomass and ethanol production in the autotrophic conditions.
本研究表明，增加 NADPH 的产生可以提高蓝藻的生物量和乙醇产量。我们在模式蓝藻集胞藻 PCC 6803 中过表达了编码戊糖磷酸途径葡萄糖-6-磷酸脱氢酶的内源 zwf 基因。zwf 的过表达导致 NADPH 的产生增加，并且与野生型相比，在自养和混合营养条件下都促进了生物量的产生。包括 NADPH 依赖的醇脱氢酶在内的乙醇生产途径也被整合到有无 zwf 过表达的情况下。zwf 过表达产生的过多 NADPH 可以提高自养条件下的生物量和乙醇产量。
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