Jiang Kai, Zhuang Ying, Yan Ming, Chen Hui, Ge An-Qi, Sun Li, Miao Bei
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China.
Laboratory of Clinical and Experimental Pathology, Xuzhou Medical College, Xuzhou 221004, China.
Neurosci Lett. 2016 Apr 8;618:127-133. doi: 10.1016/j.neulet.2016.02.065. Epub 2016 Mar 4.
Neuropathic pain is becoming an intractable health threat, with its profound effect on quality of life, thus posing a major challenge to clinical therapy. Despite the reported efficacy of riluzole in some pain models, the underlying mechanism remains largely unknown. The present study aimed to assess the effects of riluzole in a rat model of neuropathic pain induced by chronic constriction injury (CCI). Subsequent to model establishment, paw withdrawal latencies (PWLs) and the paw withdrawal mecha threshold (PWT) rapidly decreased, coupled with inhibited microglial activation and upregulated P2X7R expression in the spinal cord dorsal horn (SCDH). Following intraperitoneal administration of riluzole (4mg/kg) once daily for 5 consecutive days as from day 3 after surgery, the mechanical allodynia and thermal hyperalgesia in the hind limbs were significantly attenuated. In addition, riluzole downregulated P2X7R expression and inhibited microglial activation in SCDH. Our results indicated that riluzole is effective in alleviating neuropathic pain and inhibiting microglial activation, presumably via the downregulated P2X7R expression in SCDH.
神经性疼痛正成为一种棘手的健康威胁，对生活质量有深远影响，从而给临床治疗带来重大挑战。尽管已报道利鲁唑在某些疼痛模型中有疗效，但其潜在机制仍 largely 未知。本研究旨在评估利鲁唑在慢性压迫性损伤（CCI）诱导的神经性疼痛大鼠模型中的作用。模型建立后，爪部撤离潜伏期（PWLs）和爪部撤离机械阈值（PWT）迅速降低，同时脊髓背角（SCDH）中的小胶质细胞激活受到抑制且 P2X7R 表达上调。自手术后第 3 天起，连续 5 天每天腹腔注射一次利鲁唑（4mg/kg）后，后肢的机械性异常性疼痛和热痛觉过敏明显减轻。此外，利鲁唑下调了 SCDH 中 P2X7R 的表达并抑制了小胶质细胞激活。我们的结果表明，利鲁唑可有效减轻神经性疼痛并抑制小胶质细胞激活，可能是通过下调 SCDH 中 P2X7R 的表达来实现的。
