Ran Junjian, Sun Huadi, Zhu Mingming, Chen Juan, Zhao Ruixiang
Henan Institute of Science and Technology, School of Food Science, East Hualan Rd, Xinxiang City, Henan Province, People's Republic of China 453003.
Xinke College, Henan Institute of Science and Technology, Xinxiang, Henan, People's Republic of China 453003.
J AOAC Int. 2016 Mar-Apr;99(2):481-8. doi: 10.5740/jaoacint.15-0240. Epub 2016 Mar 4.
A method based on a reversed-phase HPLC method was established, optimized, and validated for the separation and quantitation of 10 polyphenols extracted from the peel of apple species. A bidentate reversed-phase C18 column was used as stationary phase, and an acidified water buffer and methanol were used as mobile phase. The polyphenols were well separated and detected using UV at 280 and 320 nm. Validation parameters, such as linearity, LOD, LOQ, accuracy, and precision, were acceptable for all 10 polyphenols. The proposed method has enough linearity with correlation coefficient >0.99 within the investigated range for all tested polyphenols. The LOD was 0.24 μg/mL for ellagic acid and <0.2 μg/mL for all other polyphenols. The LOQ was 9.39 × 10(-2) μg/mL for chlorogenic acid, and ellagic acid, 2.82 × 10(-2) μg/mL for caffeic acid and >0.1 μg/mL for all other polyphenols. Recovery was within the acceptable range from 98.38 to 100.39% for all polyphenols standards. Satisfactory precision was achieved for both intra- and interday assay, with RSD <2%. The method was successfully applied for simultaneous analysis of polyphenols from apple peel.
建立了一种基于反相高效液相色谱法的方法，用于从苹果品种果皮中提取的10种多酚的分离和定量，并对该方法进行了优化和验证。使用双齿反相C18柱作为固定相，酸化水缓冲液和甲醇作为流动相。通过在280和320 nm处的紫外检测，多酚得到了很好的分离。所有10种多酚的线性、检测限、定量限、准确度和精密度等验证参数均符合要求。所提出的方法在所有测试多酚的研究范围内具有足够的线性，相关系数>0.99。鞣花酸的检测限为0.24 μg/mL，其他所有多酚的检测限<0.2 μg/mL。绿原酸和鞣花酸的定量限为9.39×10(-2) μg/mL，咖啡酸的定量限为2.82×10(-2) μg/mL，其他所有多酚的定量限>0.1 μg/mL。所有多酚标准品的回收率在98.38%至100.39%的可接受范围内。日内和日间测定均获得了满意的精密度，相对标准偏差<2%。该方法成功应用于苹果皮中多酚的同时分析。
