波兰肉鸡群中鸡贫血病毒的发生情况及系统发育研究。

Occurrence and Phylogenetic Studies of Chicken Anemia Virus from Polish Broiler Flocks.

作者信息

Olszewska-Tomczyk Monika, Świętoń Edyta, Minta Zenon, Śmietanka Krzysztof

机构信息

National Veterinary Research Institute, Department of Poultry Diseases, Al. Partyzantów 57, 24-100 Puławy, Poland.

出版信息

Avian Dis. 2016 Mar;60(1):70-4. doi: 10.1637/11277-091415-ResNote.1.

DOI:10.1637/11277-091415-ResNote.1

PMID:26953947

Abstract

Chicken anemia virus (CAV) is a widespread chicken pathogen of significant economic importance. In 2013, broiler chicken flocks in Poland were examined for the presence of CAV, and phylogenetic relatedness between the strains was established. Ten cloacal swabs from each of 106 broiler flocks (birds aged 3-6 wk) were collected in different regions of the country and tested with the use of real-time PCR (all samples) and conventional PCR (those samples positive in real-time PCR) assays. The presence of CAV was detected in 16 of the flocks tested. Phylogenetic analysis clearly confirmed the existence of genetic diversity within the group of circulating CAV strains and their distinctiveness from vaccine strains used in Poland.

摘要

鸡贫血病毒（CAV）是一种广泛存在且具有重大经济意义的鸡病原体。2013年，对波兰的肉鸡群进行了CAV检测，并确定了各毒株之间的系统发育相关性。从该国不同地区的106个肉鸡群（3至6周龄的鸡）中，每个鸡群采集10份泄殖腔拭子，使用实时荧光定量PCR（所有样本）和常规PCR（实时荧光定量PCR检测呈阳性的样本）检测方法进行检测。在检测的鸡群中，有16个鸡群检测到CAV。系统发育分析清楚地证实了流行的CAV毒株群体内存在遗传多样性，且它们与波兰使用的疫苗毒株不同。