养阴清肺汤对干燥综合征模型小鼠的干预作用

[Intervention of Yangfei Ziyin Decoction on Sjogren's Syndrome Model Mice].

作者信息

Tian Meng-jiang, Tu Zhia-hua, Hu Rui, Zhu Xuan-xuan

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Jan;36(1):63-8.

Abstract

OBJECTIVE

To observe the effect of Yangfei Ziyin Decoction (YZD) on symptoms, serum levels of TNF-alpha, IL-6, and aquaporin-5 (AQP-5), and pathology of Sj6gren's syndrome (SS) model mice.

METHODS

Totally 60 mice were divided into 6 groups according to random digit table, i.e., the model group, the normal control group, the high, medium, low dose YZD groups (administered with YZD at 36.7, 18.4, 9.2 g/kg, 0.2 mL/10 g), the Chinese patent medicine group [CPM, administered with total glucosides of paeony at 0.6 g/kg], 10 mice in each group. All intervention was performed for six successive days in a week, with an interval of one day, a total of 50 days. Body weight, salivary secretion, food and water intake were measured at day10, 20, 30, 40, and 50, respectively. At day 50 blood was collected. Submandibular gland, thymus, and spleen were weighed. Serum levels of TNF-alpha, IL-6, and AQP-5 were detected by ELISA. Pathological changes of submandibular gland were observed. Results Compared with the normal control group, there was no change in water intake of mice in the model group, but with reduced salivary secretion (P < 0.01, P < 0.05). Thymus/spleen/submandibular gland weight and index increased in the model group (P < 0.01, P < 0.05). Compared with the model group at the same time point, salivary secretion increased in the CPM group and 3 YZD groups (P < 0.01 , P < 0.05). Compared with the model group, thymus/spleen/submandibular gland weight and index decreased in the CPM group (P < 0.01, P < 0.05). Thymus/submandibular gland weight and index decreased in the low dose YZD group (P < 0.01, P < 0.05). Thymus/submandibular gland weight and index, and spleen index decreased in high and medium dose YZD groups (P < 0.01 , P < 0.05). Levels of TNF-alpha and IL-6 decreased, but AQP-5 level increased in the CPM group (P < 0.05). AQP-5 level increased in high and medium dose YZD groups (P < 0.01 , P < 0.05). In the model group alveoli and duct of salivary gland were destroyed, alveoli and duct were irregular, epithelial cells were degenerated, necrotic, and desquamated. Mild-to-moderate lymphocytic infiltration occurred around submandibular gland. Pathological changes were alleviated in the CPM group and 3 YZD groups.

CONCLUSION

YZD could improve clinical symptoms, serum levels of TNF-alpha, IL-6, AQP-5, and pathological changes of SS model mice.

摘要

目的

观察养肺滋阴汤（YZD）对干燥综合征（SS）模型小鼠症状、血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）及水通道蛋白-5（AQP-5）水平和病理变化的影响。

方法

将60只小鼠按随机数字表法分为6组，即模型组、正常对照组、YZD高、中、低剂量组（分别按36.7、18.4、9.2 g/kg，0.2 mL/10 g给予YZD）、中成药组[CPM，按0.6 g/kg给予白芍总苷]，每组10只。所有干预均每周连续进行6天，间隔1天，共50天。分别在第10、20、30、40和50天测量体重、唾液分泌量、食物和水摄入量。在第50天采集血液。称量下颌下腺、胸腺和脾脏重量。采用酶联免疫吸附测定（ELISA）法检测血清TNF-α、IL-6和AQP-5水平。观察下颌下腺的病理变化。结果与正常对照组相比，模型组小鼠饮水量无变化，但唾液分泌减少（P<0.01，P<0.05）。模型组胸腺/脾脏/下颌下腺重量及指数增加（P<0.01，P<0.05）。与同时间点模型组相比，CPM组和3个YZD组唾液分泌增加（P<0.01，P<0.05）。与模型组相比，CPM组胸腺/脾脏/下颌下腺重量及指数降低（P<0.01，P<0.05）。低剂量YZD组胸腺/下颌下腺重量及指数降低（P<0.01，P<0.05）。高、中剂量YZD组胸腺/下颌下腺重量及指数和脾脏指数降低（P<0.01，P<0.05）。CPM组TNF-α和IL-6水平降低，但AQP-5水平升高（P<0.05）。高、中剂量YZD组AQP-5水平升高（P<0.01，P<0.05）。模型组唾液腺腺泡和导管破坏，腺泡和导管不规则，上皮细胞变性、坏死及脱落。下颌下腺周围有轻至中度淋巴细胞浸润。CPM组和3个YZD组病理变化减轻。