红景天苷对抽动秽语综合征模型大鼠抽动行为的影响

[Effects of Salidroside on Tic Behavior of Tourette Syndrome Model Rats].

作者信息

Xie Hui, Wang Zhen, Ji Yan, Yin Jing, Yang Wen-hao, Ren Li-min

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Jan;36(1):90-3.

Abstract

OBJECTIVE

To observe the effect of salidroside on tic behavior and in vivo dopamine DA) and serotonin (5-HT) levels in Tourette syndrome (TS) model rats.

METHODS

Forty rats were randomly divided into the blank control group, the TS model group, the haloperidol-treated group (0.5 mg/kg x d(-1)), and the salidroside-treated group (50 mg/kg x d(-1)), 10 in each group. TS rat model was induced by imino-dipropio-nitrile (IDPN). Peritoneal injection of haloperidol and salidroside was started from the 4th day of modeling in the haloperidol-treated group and the salidroside-treated group respectively. Normal saline was peritoneally injected to rats in the blank control group and the TS model group respectively. Stereotyped behavior was scored, and changes of DA and 5-HT levels in blood and striatum were measured before modeling, after modeling, and after intervention.

RESULTS

Compared with the blank control group, the score of the tic behavior was elevated (P < 0.01) , levels of DA and 5-HT in plasma and striatum were reduced in the model group (P < 0.01, P < 0.05). Compared with the same group after modeling, the tic behavior score decreased and plasma DA levels increased in the two treated groups after intervention (P < 0.01). 5-HT content increased in the salidroside-treated group (P < 0.01). Compared with the model group after intervention, the tic behavior score was significantly reduced (P < 0.01), and DA levels in plasma and striatum were elevated (P < 0.01, P < 0.05) in the salidroside-treated group and the haloperidol-treated group. Compared with the haloperidol-treated group, the tic behavior score increased (P < 0.01), DA levels in plasma and striatum were lowered (P < 0.01, P < 0.05), the 5-HT level increased in plasma and striatum (P < 0.01, P < 0.05) in the salidroside-treated group.

CONCLUSIONS

In the salidroside-treated group, the tic behavior was significantly reduced, and DA levels in plasma and striatum were elevated. Its mechanism might be related to regulating activities of dopamine neurons in striatum.

摘要

目的

观察红景天苷对妥瑞氏综合征（TS）模型大鼠抽动行为及体内多巴胺（DA）和5-羟色胺（5-HT）水平的影响。

方法

将40只大鼠随机分为空白对照组、TS模型组、氟哌啶醇治疗组（0.5mg/kg×d⁻¹）和红景天苷治疗组（50mg/kg×d⁻¹），每组10只。采用亚氨基二丙腈（IDPN）诱导建立TS大鼠模型。氟哌啶醇治疗组和红景天苷治疗组分别于造模第4天开始腹腔注射氟哌啶醇和红景天苷。空白对照组和TS模型组大鼠分别腹腔注射生理盐水。对刻板行为进行评分，并在造模前、造模后及干预后检测血液和纹状体中DA和5-HT水平的变化。

结果

与空白对照组相比，模型组抽动行为评分升高（P<0.01），血浆和纹状体中DA和5-HT水平降低（P<0.01，P<0.05）。与造模后同组相比，干预后两个治疗组的抽动行为评分降低，血浆DA水平升高（P<0.01）。红景天苷治疗组5-HT含量增加（P<0.01）。与干预后模型组相比，红景天苷治疗组和氟哌啶醇治疗组的抽动行为评分显著降低（P<0.01），血浆和纹状体中DA水平升高（P<0.01，P<0.05）。与氟哌啶醇治疗组相比，红景天苷治疗组的抽动行为评分升高（P<0.01），血浆和纹状体中DA水平降低（P<0.01，P<0.05），血浆和纹状体中5-HT水平升高（P<0.01，P<0.05）。