Matayoshi S, Oda H, Sarwar G
Department of Bacteriology, Faculty of Medicine, Kagoshima University, Japan.
J Gen Microbiol. 1989 Mar;135(3):525-9. doi: 10.1099/00221287-135-3-525.
The effects of culture conditions (aerobic or anaerobic) and glucose in the medium on the production of spirosomes in Escherichia coli B were studied by SDS-PAGE and electron microscopy. The Mr of the spirosome of E. coli B was estimated to be 97,000. Electron microscopy revealed that the amount of spirosomes derived from anaerobic cultures was about eightfold larger than that from aerobic cultures. In SDS-PAGE, the bands of spirosome protein derived from anaerobic cultures were more intense than those derived from aerobic cultures, either in peptone water or in Davis-Mingioli's minimal medium. With increased glucose concentration under aerobic conditions, the intensity of the band of spirosome protein was similar to that observed under anaerobic conditions in basal media. These results suggest that spirosome production by E. coli B is related to its anaerobic glycolysis activity.
通过SDS - PAGE和电子显微镜研究了培养条件（需氧或厌氧）以及培养基中的葡萄糖对大肠杆菌B中螺旋体产生的影响。大肠杆菌B螺旋体的相对分子质量估计为97,000。电子显微镜显示，厌氧培养产生的螺旋体数量比需氧培养产生的大约多八倍。在SDS - PAGE中，无论是在蛋白胨水还是在戴维斯 - 明乔利基本培养基中，厌氧培养产生的螺旋体蛋白条带都比需氧培养产生的更密集。在需氧条件下，随着葡萄糖浓度的增加，螺旋体蛋白条带的强度与基础培养基中厌氧条件下观察到的相似。这些结果表明，大肠杆菌B产生螺旋体与其厌氧糖酵解活性有关。
