尼日利亚西南部铜绿假单胞菌临床分离株中氨基糖苷类修饰酶基因（aac(6')-I和ant(2″)-I）的出现情况
Occurrence of aminoglycoside-modifying enzymes genes (aac(6')-I and ant(2″)-I) in clinical isolates of Pseudomonas aeruginosa from Southwest Nigeria.
作者信息
Odumosu Bamidele Tolulope, Adeniyi Bolanle A, Chandra Ram
机构信息
Department of Microbiology University of Lagos, Akoka Lagos; Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria; Environmental Microbiology Section, Indian Institute of Toxicology Research, Lucknow, India.
Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.
出版信息
Afr Health Sci. 2015 Dec;15(4):1277-81. doi: 10.4314/ahs.v15i4.29.
BACKGROUND
Enzymatic modification of aminoglycosides is the primary mechanism of resistance by Pseudomonas aeruginosa.
OBEJECTIVES
We investigated the occurrence and mechanism of aminoglycosides resistance in P. aeruginosa isolates from hospitals in SouthWest Nigeria.
METHODS
A total of 54 consecutive, non-duplicate clinical isolates of P. aeruginosa were studied for the presence of aminoglycosides -modifying enzymes (AMEs) by PCR amplification and sequencing of genes encoding AMEs.
RESULTS AND CONCLUSION
Two types of AME genes [aac (6') - I and ant (2″) - I] were found in 12 isolates out of 54. Seven strains harboured one or more types of enzymes of which aac (6') - I was the most frequently found gene (10/54 isolates, 18.5%). None of the isolates investigated in this study were positive for aph, aac (3) and aac (6″) - II genes. Prevalence of P. aeruginosa producing AME genes in this study may suggest aminoglycosides use in Nigeria. This study highlights need for functional antimicrobial surveillance system in Nigeria.
背景
氨基糖苷类药物的酶促修饰是铜绿假单胞菌耐药的主要机制。
目的
我们调查了尼日利亚西南部医院分离的铜绿假单胞菌中氨基糖苷类耐药的发生情况及机制。
方法
通过对编码氨基糖苷类修饰酶（AMEs）的基因进行PCR扩增和测序，研究了54株连续的、非重复的铜绿假单胞菌临床分离株中AMEs的存在情况。
结果与结论
在54株分离株中的12株中发现了两种类型的AME基因[aac(6')-I和ant(2″)-I]。7株菌株携带一种或多种类型的酶，其中aac(6')-I是最常发现的基因（10/54株分离株，18.5%）。本研究中调查的分离株均未检测到aph、aac(3)和aac(6″)-II基因呈阳性。本研究中产生AME基因的铜绿假单胞菌的流行情况可能提示尼日利亚存在氨基糖苷类药物的使用。本研究强调了尼日利亚建立功能性抗菌药物监测系统的必要性。
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