Liu Chao, Zhang Dijuan, Shen Yuxian, Tao Xiaofang, Liu Lihua, Zhong Yongwang, Fang Shengyun
Department of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, China; Center for Biomedical Engineering and Technology (BioMET), University of Maryland, Baltimore, MD 21201, USA.
Department of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, China.
Data Brief. 2015 Oct 19;5:599-604. doi: 10.1016/j.dib.2015.10.010. eCollection 2015 Dec.
DPF2, also named ubi-d4/requiem (REQU), interacts with a protein complex containing OCT4. This paper provides data in support of the research article entitled "DPF2 regulates OCT4 protein level and nuclear distribution". The highlights include: (1) Denature-immunoprecipitation assay revealed ubiquitination of OCT4 in pluripotent H9 cells, which was enhancedby MG132, a proteasome inhibitor. (2) Well colocalization of ectopic OCT4 and FLAG-Ub was found in HeLa cells, which was also increased by MG132. (3) MG132 treatment decreased DPF2 cytoplasmic expression in vivo. These data give insights into how proteasome inhibition contributes to studying ubiquitnation of OCT4.
DPF2，也被称为泛素-d4/安魂曲（REQU），与包含OCT4的蛋白质复合物相互作用。本文提供的数据支持了题为“DPF2调节OCT4蛋白水平和核分布”的研究文章。要点包括：（1）变性免疫沉淀试验揭示了多能性H9细胞中OCT4的泛素化，蛋白酶体抑制剂MG132增强了这种泛素化。（2）在HeLa细胞中发现异位OCT4和FLAG-Ub有良好的共定位，MG132也增加了这种共定位。（3）MG132处理在体内降低了DPF2的细胞质表达。这些数据为蛋白酶体抑制如何有助于研究OCT4的泛素化提供了见解。
