Shimizu Makiko, Koyama Tomoki, Kishimoto Izumi, Yamazaki Hiroshi
Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan.
Data Brief. 2015 Oct 25;5:642-5. doi: 10.1016/j.dib.2015.10.019. eCollection 2015 Dec.
This data article contains a supplementary figure and validation data relating to the research article entitled "Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain reaction method with dual-labeled probes" (Shimizu et al., Clinica Chimica Acta 441, 71-74, 2015), which presents a multiplex real-time polymerase chain reaction method with dual-labeled probes for human P450 2A6 wild-type and whole-gene deletion. Real-time methods have dramatically improved the speed of complex genetic diagnostics compared to conventional assays based on restriction enzyme digestion. Here, we show the basic assay validation data by single and multiplex determinations in comparison with commercial TaqMan copy number assays for P450 2A6.
本数据文章包含与题为《使用人血样本和带有双标记探针的多重实时聚合酶链反应方法对野生型细胞色素P450 2A6进行基因分型和全基因缺失检测》（清水等人，《临床化学学报》441卷，第71 - 74页，2015年）的研究文章相关的补充图和验证数据，该研究文章介绍了一种用于人P450 2A6野生型和全基因缺失检测的带有双标记探针的多重实时聚合酶链反应方法。与基于限制性酶切的传统检测方法相比，实时方法极大地提高了复杂基因诊断的速度。在此，我们展示了通过单重和多重测定获得的基本检测验证数据，并与用于P450 2A6的商业TaqMan拷贝数检测方法进行了比较。
