Ling King-Hwa, Brautigan Peter J, Moore Sarah, Fraser Rachel, Leong Melody Pui-Yee, Leong Jia-Wen, Zainal Abidin Shahidee, Lee Han-Chung, Cheah Pike-See, Raison Joy M, Babic Milena, Lee Young Kyung, Daish Tasman, Mattiske Deidre M, Mann Jeffrey R, Adelson David L, Thomas Paul Q, Hahn Christopher N, Scott Hamish S
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia; NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia.
Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
Data Brief. 2016 Feb 17;7:282-90. doi: 10.1016/j.dib.2016.01.045. eCollection 2016 Jun.
SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1], [2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1.
SRY（Y染色体性别决定区）-盒4，即Sox4，是哺乳动物大脑有丝分裂后细胞分化过程中泛神经元基因表达的重要调节因子。Sox4基因位点先前已被表征为具有多个正义和重叠的天然反义转录本[1,2]。在此，我们提供了Ling等人[2]所进行和描述的各种分析的相关数据。这些数据包括在Sox4基因位点发现的各种特征的详细描述、源自RNA荧光原位杂交（RNA-FISH）、蛋白质免疫印迹、链特异性逆转录定量聚合酶链反应（RT-qPCR）、功能获得和原位杂交（ISH）实验的额外实验数据。这里提供的所有额外数据都支持一种内源性小干扰RNA或类PIWI相互作用小RNA（称为Sox4_sir3）的存在，其起源于由一个正义转录本和一个称为Sox4ot1的天然反义转录本组成的重叠区域内。
