完全基于碳水化合物的桑森-弗里德赖希抗原 - 肺炎链球菌23F型多糖结合物的免疫学评估。
Immunological evaluation of the entirely carbohydrate-based Thomsen-Friedenreich - PS B conjugate.
作者信息
Trabbic Kevin R, Bourgault Jean-Paul, Shi Mengchao, Clark Matthew, Andreana Peter R
机构信息
Department of Chemistry and Biochemistry, University of Toledo, Wolfe Hall, Room 2232B, 2801 W. Bancroft Street, Toledo, OH 43606-3390, USA.
出版信息
Org Biomol Chem. 2016 Apr 7;14(13):3350-5. doi: 10.1039/c6ob00176a. Epub 2016 Mar 9.
PS B, a naturally occurring CD4(+) T-cell simulating zwitterionic polysaccharide from Bacteroides fragilis ATCC 25285/NCTC 9343, was conjugated with aminooxy Thomsen Friedenreich (TF or T) [α-d-Gal-(1,3)-β-d-GalNAc-ONH2] tumor antigen. Immunization in Jax C57BL/6, followed by ELISA revealed IgM and IgG antibody TF specificity. FACS data noted preferential binding to TF-laced MCF-7 cells but not to HCT-116 cells.
PS B是一种从脆弱拟杆菌ATCC 25285/NCTC 9343中天然存在的模拟两性离子多糖的CD4(+) T细胞，它与氨氧基汤姆森·弗里德赖希（TF或T）[α-d-半乳糖-(1,3)-β-d- N-乙酰半乳糖胺-ONH2]肿瘤抗原偶联。在Jax C57BL/6小鼠中进行免疫，随后通过酶联免疫吸附测定法显示出IgM和IgG抗体对TF具有特异性。荧光激活细胞分选术数据表明其优先结合含有TF的MCF-7细胞，而不结合HCT-116细胞。
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