Chen Yun-jie, Jiang Hai-tao, Cao Jing-yu
Department of General Surgery, Ningbo No. 2 Hospital, Ningbo 315010, Zhejiang, China.
Department of Hepatobiliary Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China.
Chin Med Sci J. 2015 Dec;30(4):252-9. doi: 10.1016/s1001-9294(16)30009-8.
To investigate the effect of photodynamic therapy (PDT) mediated by hematoporphyrin derivative (HPD) on apoptosis and invasion of cholangiocarcinoma QBC939 cell lines.
In vitro cultured cholangiocarcinoma QBC939 cell line was exposed to 2, 4, 6, 8, 10, 12, and 14 μg/ml HPD with 5, 10, and 15 J/cm2 light intensity, respectively. The optical density at 450 nm of the QBC939 cells was measured by CCK8 assay and its growth inhibition ratio was calculated. Flow cytometry and transwell migration assay were applied to detect cell apoptosis and invasion respectively. RT-PCR and immunocytochemistry analyses were used to detect expressions of vascular endothelial growth factor-C (VEGF-C), cyclooxygenase-2 (COX-2), and proliferating cell nuclear antigen (PCNA). Enzyme-linked immunosorbent assay (ELISA) was carried out to examine the secretion of VEGF-C and COX-2 in QBC939 cells.
Exposure to HPD-PDT can significantly suppress the growth of QBC939 cells (all P<0.05). HPD-PDT can promote apoptosis of QBC939 cells at the early stage. When the concentration of HPD was 2 μg/ml and light irradiation was 5 J/cm2, HPD-PDT had no obvious inhibitory effect on QBC939 cell growth, but can obviously inhibit cell invasion, and significant difference was observed between the HPD-PDT and control groups (P<0.01). The HPD-PDT can reduce the mRNA and protein expressions of VEGF-C, COX-2, and PCNA, and decrease the secretion of VEGF-C and COX-2 in QBC939 cells.
PDT could promote apoptosis and inhibit growth and invasion of cholangiocarcinoma cells QBC939 in vitro.
探讨血卟啉衍生物（HPD）介导的光动力疗法（PDT）对胆管癌QBC939细胞系凋亡及侵袭的影响。
体外培养胆管癌QBC939细胞系，分别用2、4、6、8、10、12和14μg/ml的HPD处理，光强度分别为5、10和15J/cm²。采用CCK8法检测QBC939细胞在450nm处的吸光度，并计算其生长抑制率。应用流式细胞术和Transwell迁移实验分别检测细胞凋亡和侵袭情况。采用RT-PCR和免疫细胞化学分析检测血管内皮生长因子-C（VEGF-C）、环氧合酶-2（COX-2）和增殖细胞核抗原（PCNA）的表达。采用酶联免疫吸附测定（ELISA）检测QBC939细胞中VEGF-C和COX-2的分泌情况。
HPD-PDT处理可显著抑制QBC939细胞的生长（均P<0.05）。HPD-PDT可促进QBC939细胞早期凋亡。当HPD浓度为2μg/ml且光照射强度为5J/cm²时，HPD-PDT对QBC939细胞生长无明显抑制作用，但可明显抑制细胞侵袭，HPD-PDT组与对照组之间差异有统计学意义（P<0.01）。HPD-PDT可降低QBC939细胞中VEGF-C、COX-2和PCNA的mRNA及蛋白表达，并减少VEGF-C和COX-2的分泌。
PDT在体外可促进胆管癌细胞QBC939凋亡，抑制其生长和侵袭。
