Chen Yansu, Huang Yefei, Zhu Liwen, Chen Minjuan, Huang Yulin, Zhang Jianbing, He Song, Li Aiping, Chen Rui, Zhou Jianwei
Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.
School of Public Health, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, 221002, People's Republic of China.
J Cancer Res Clin Oncol. 2016 Jun;142(6):1221-30. doi: 10.1007/s00432-016-2125-4. Epub 2016 Mar 9.
To investigate the potential role of SOX2 in gastric cancer (GC) metastasis.
The SOX2 expression was detected using immunohistochemistry on a GC tissue microarray. The correlations of SOX2 expression with clinicopathological factors and 5-year survival were evaluated. To test the role of SOX2 in inhibiting GC metastasis, the cell transwell assay was performed. Real-time PCR and Western blot were used to explore the possible mechanism that SOX2 inhibits GC metastasis.
In the present study, SOX2 expression was downregulated in GC tissues when compared to matching normal tissues. Moreover, patients with high SOX2 expression in cancerous tissues had less lymph node metastasis and better treatment outcome. At the subcellular level, SOX2 inhibited the GC cell migration and invasion by upregulating p21 expression. Moreover, SOX2 was determined to associate with the nuclear p21 expression. GC patients with high SOX2 and nuclear p21 expression had synergistically less lymph node metastasis and the better overall survival.
Our results suggest that SOX2 is a promising and favorable metastatic biomarker for GC.
探讨SOX2在胃癌（GC）转移中的潜在作用。
采用免疫组织化学法在胃癌组织芯片上检测SOX2表达。评估SOX2表达与临床病理因素及5年生存率的相关性。为检测SOX2在抑制胃癌转移中的作用，进行了细胞Transwell实验。采用实时荧光定量PCR和蛋白质免疫印迹法探讨SOX2抑制胃癌转移的可能机制。
在本研究中，与配对的正常组织相比，胃癌组织中SOX2表达下调。此外，癌组织中SOX2高表达的患者淋巴结转移较少，治疗效果较好。在亚细胞水平上，SOX2通过上调p21表达抑制胃癌细胞迁移和侵袭。此外，SOX2被确定与细胞核p21表达相关。SOX2和细胞核p21高表达的胃癌患者淋巴结转移协同减少，总生存率更高。
我们的结果表明，SOX2是一种有前景且良好的胃癌转移生物标志物。
