Xu Yanbing, Zheng Zhaojuan, Xu Qianqian, Yong Qiang, Ouyang Jia
Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education , Nanjing, People's Republic of China.
J Agric Food Chem. 2016 Mar 30;64(12):2612-8. doi: 10.1021/acs.jafc.5b05908. Epub 2016 Mar 21.
Inulooligosaccharides (IOS) represent an important class of oligosaccharides at industrial scale. An efficient conversion of inulin to IOS through endoinulinase from Aspergillus niger is presented. A 1482 bp codon optimized gene fragment encoding endoinulinase from A. niger DSM 2466 was cloned into pPIC9K vector and was transformed into Pichia pastoris KM71. Maximum activity of the recombinant endoinulinase, 858 U/mL, was obtained at 120 h of the high cell density fermentation process. The optimal conditions for inulin hydrolysis using the recombinant endoinulinase were investigated. IOS were harvested with a high concentration of 365.1 g/L and high yield up to 91.3%. IOS with different degrees of polymerization (DP, mainly DP 3-6) were distributed in the final reaction products.
低聚菊糖（IOS）是工业规模上一类重要的低聚糖。本文介绍了通过黑曲霉的内切菊粉酶将菊粉高效转化为IOS的过程。将一个1482 bp的编码黑曲霉DSM 2466内切菊粉酶的密码子优化基因片段克隆到pPIC9K载体中，并转化到毕赤酵母KM71中。在高细胞密度发酵120 h时，重组内切菊粉酶的最大活性达到858 U/mL。研究了使用重组内切菊粉酶水解菊粉的最佳条件。收获的IOS浓度高达365.1 g/L，产率高达91.3%。不同聚合度（DP，主要为DP 3-6）的IOS分布在最终反应产物中。
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