Li Xin, Zhang Qiannan, Wang Wei, Yang Shang-Tian
Jiangsu Co-innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
Jiangsu Province Key Laboratory of Green Biomass-Based Fuels and Chemicals, Nanjing, China.
Front Bioeng Biotechnol. 2021 Apr 29;9:679720. doi: 10.3389/fbioe.2021.679720. eCollection 2021.
Inulin is a kind of polysaccharide that can be obtained various biomass. Inulooligosaccharides (IOS), a kind of oligosaccharides that can be obtained from inulin by enzymatic hydrolysis using inulinases, have been regarded as the functional food ingredients. Commercially available inulinases produced by natural contained both endo- and exo-inulinase activities. For IOS production from inulin, it is desirable to use only endo-inulinase as exo-inulinase would produce mainly the monosacchairde fructose from inulin. In the present study, a simple inulin-mediated ethanol precipitation method was developed to separate endo- and exo-inulinases present in natural inulinases. IOS production from inulin using the enriched endo-inulinase was then optimized in process conditions including pH and temperature, achieving a high yield of ∼94%. The resultant IOS products had a degree of polymerization ranging from 2 to 7. The study demonstrated a novel method for obtaining partially purified or enriched endo-inulinase for IOS production from inulin in an efficient process.
菊粉是一种可从多种生物质中获得的多糖。低聚菊粉（IOS）是一种可通过使用菊粉酶进行酶水解从菊粉中获得的低聚糖，被视为功能性食品成分。天然生产的市售菊粉酶同时具有内切菊粉酶和外切菊粉酶活性。对于从菊粉生产IOS而言，仅使用内切菊粉酶是理想的，因为外切菊粉酶会主要从菊粉产生单糖果糖。在本研究中，开发了一种简单的菊粉介导的乙醇沉淀方法，以分离天然菊粉酶中存在的内切菊粉酶和外切菊粉酶。然后在包括pH和温度在内的工艺条件下优化了使用富集的内切菊粉酶从菊粉生产IOS的过程，实现了约94%的高产率。所得的IOS产品的聚合度范围为2至7。该研究展示了一种在高效过程中从菊粉获得用于生产IOS的部分纯化或富集内切菊粉酶的新方法。
