Wang Gang, Li Li, Yu Ying, Tu Yabin, Tong Jie, Zhang Chong, Liu Yonggang, Li Yuming, Han Zifeng, Jiang Chenggang, Wang Shujie, Zhou En-Min, He Xijun, Cai Xuehui
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150001, PR China.
Jilin Agricultural University, Changchun, Jilin Province 130118, PR China.
J Gen Virol. 2016 Jun;97(6):1356-1361. doi: 10.1099/jgv.0.000454. Epub 2016 Mar 10.
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has been shown to have a wide range of tissue tropism, and can directly and indirectly induce cellular apoptosis. However, the impact of HP-PRRSV infection on the bone marrow (BM) of piglets remains unclear. In this study, we investigated the BM as a novel site of infection by the HP-PRRSV strain in piglets. HP-PRRSV infected SWC3+SWC8- cells in the BM and induced BM cells to undergo apoptosis. The number of apoptotic cells highlights the striking effects of HP-PRRSV on the central immune organs (BM and thymus) that may enhance the susceptibility of pigs to secondary infections and lead to high mortality. This study is, to the best of our knowledge, the first to report the impact of HP-PRRSV on the BM and implicate the depletion of BM cells during HP-PRRSV infection in the development of immunosuppression in this disease.
高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV）已被证明具有广泛的组织嗜性，并且能够直接和间接诱导细胞凋亡。然而，HP-PRRSV感染对仔猪骨髓（BM）的影响仍不清楚。在本研究中，我们调查了仔猪骨髓作为HP-PRRSV毒株新感染位点的情况。HP-PRRSV感染了骨髓中的SWC3+SWC8-细胞，并诱导骨髓细胞发生凋亡。凋亡细胞的数量突出了HP-PRRSV对中枢免疫器官（骨髓和胸腺）的显著影响，这可能会增强猪对继发感染的易感性并导致高死亡率。据我们所知，本研究首次报道了HP-PRRSV对骨髓的影响，并暗示在该疾病的免疫抑制发展过程中，HP-PRRSV感染期间骨髓细胞的耗竭。
