Nilsson-Ehle I
Acta Pathol Microbiol Scand Suppl. 1977(259):61-6.
The toxicity of some antibiotics used today necessitates rapid, specific and accurate determinations of antibiotic concentrations in biological fluids. Microbiological assays do not fulfill these needs and new procedures have recently been developed, for instance, fluorimetric assays, radioimmunoassays and radioenzymatic assays. However, these techniques have not been generally applicable to various antimicrobial agents. - The technique for high-pressure liquid chromatography (HPLC) has recently been applied to determination of antibiotics in biological fluids. The methods involve extraction of drug from the biological samples, separation by HPLC and detection by ultraviolet spectrophotometry of fluorimetry. Results obtained with tetracycline, amphotericin B, and cephalothin show that this procedure meets the demands for rapid, specific and accurate monitoring of antibiotic concentrations in body fluids. Because of its versatility, the HPLC technique seems to be applicable to the determination of a variety of antibiotics and other drugs in clinical and experimental medicine.
当今使用的一些抗生素具有毒性，因此需要快速、特异且准确地测定生物体液中的抗生素浓度。微生物学检测无法满足这些需求，最近已开发出一些新方法，例如荧光测定法、放射免疫测定法和放射酶测定法。然而，这些技术尚未普遍适用于各种抗菌剂。
高压液相色谱法（HPLC）最近已应用于生物体液中抗生素的测定。这些方法包括从生物样品中提取药物、通过HPLC进行分离以及通过紫外分光光度法或荧光法进行检测。四环素、两性霉素B和头孢噻吩的检测结果表明，该方法满足了快速、特异且准确地监测体液中抗生素浓度的要求。由于其通用性，HPLC技术似乎适用于临床和实验医学中各种抗生素及其他药物的测定。
