卵菌纲腐皮镰刀菌微卫星标记的开发与特性分析
Development and characterization of microsatellite markers for the oomyceta Aphanomyces euteiches.
作者信息
Mieuzet Lucie, Quillévéré A, Pilet M L, Le May C
机构信息
INRA, UMR1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Domaine de la Motte, 35653 Le Rheu, France; Agrocampus Ouest, Laboratoire Ecologie et Santé des Plantes (ESP), 65 rue de Saint Brieuc, 35042 Rennes, France.
INRA, UMR1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Domaine de la Motte, 35653 Le Rheu, France; Agrocampus Ouest, Laboratoire Ecologie et Santé des Plantes (ESP), 65 rue de Saint Brieuc, 35042 Rennes, France; Université de Rennes I, 9 rue Jean Macé, 35700 Rennes, France.
出版信息
Fungal Genet Biol. 2016 Jun;91:1-5. doi: 10.1016/j.fgb.2016.03.001. Epub 2016 Mar 7.
Aphanomyces euteiches Drechsler is a serious pathogen of leguminous crops that causes devastating root rot of pea worldwide. Given that A. euteiches is a diploid organism, robust, codominant markers are needed for population genetics studies. We have developed and screened a microsatellite-enriched small-insert genomic library for identification of A. euteiches SSR containing sequences. Fourteen out of the 48 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of 94 isolates. The number of alleles at each locus ranged from one to four. The identification of new markers would enhance the ability to evaluate the genetic structure of A. euteiches populations, and pathogen evolution.
掘氏疫霉（Aphanomyces euteiches Drechsler）是豆科作物的一种严重病原菌，在全球范围内导致豌豆毁灭性根腐病。鉴于掘氏疫霉是一种二倍体生物，群体遗传学研究需要强大的共显性标记。我们开发并筛选了一个富含微卫星的小插入基因组文库，以鉴定含有掘氏疫霉简单序列重复（SSR）的序列。在设计用于扩增SSR的48对引物中，有14对在我们94个分离株的测试群体中产生了明确的多态性产物。每个位点的等位基因数量从1到4不等。新标记的鉴定将增强评估掘氏疫霉群体遗传结构和病原菌进化的能力。
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