Zhao Xiaoman, Bansode Sneha Ramrao, Ribeiro Artur, Abreu Ana S, Oliveira César, Parpot Pier, Gogate P R, Rathod V K, Cavaco-Paulo Artur
Jiangsu Engineering Technology Research Center for Functional Textiles, Jiangnan University, Wuxi 214122, China; International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China.
Department of Chemical Engineering, The Institute of Chemical Technology, Mumbai 400019, India.
Ultrason Sonochem. 2016 Jul;31:506-11. doi: 10.1016/j.ultsonch.2016.02.005. Epub 2016 Feb 3.
The present work explores the best conditions for the enzymatic synthesis of poly (ethylene glutarate) for the first time. The start-up materials are the liquids; diethyl glutarate and ethylene glycol diacetate, without the need of addition of extra solvent. The reactions are catalyzed by lipase B from Candida antarctica immobilized on glycidyl methacrylate-ter-divinylbenzene-ter-ethylene glycol dimethacrylate at 40°C during 18h in water bath with mechanical stirring or 1h in ultrasonic bath followed by 6h in vacuum in both the cases for evaporation of ethyl acetate. The application of ultrasound significantly intensified the polyesterification reaction with reduction of the processing time from 24h to 7h. The same degree of polymerization was obtained for the same enzyme loading in less time of reaction when using the ultrasound treatment. The degree of polymerization for long-term polyesterification was improved approximately 8-fold due to the presence of sonication during the reaction. The highest degree of polymerization achieved was 31, with a monomer conversion of 96.77%. The ultrasound treatment demonstrated to be an effective green approach to intensify the polyesterification reaction with enhanced initial kinetics and high degree of polymerization.
本研究首次探索了酶法合成聚戊二酸乙二酯的最佳条件。起始原料为液体戊二酸二乙酯和乙二醇二乙酸酯，无需添加额外溶剂。反应由固定在甲基丙烯酸缩水甘油酯 - 三 - 二乙烯基苯 - 三 - 乙二醇二甲基丙烯酸酯上的南极假丝酵母脂肪酶B催化，在40°C下于水浴中机械搅拌18小时，或在超声浴中反应1小时，然后在两种情况下均在真空中蒸发乙酸乙酯6小时。超声的应用显著增强了聚酯化反应，将处理时间从24小时缩短至7小时。在相同的酶负载量下，使用超声处理时在更短的反应时间内获得了相同的聚合度。由于反应过程中存在超声处理，长期聚酯化的聚合度提高了约8倍。达到的最高聚合度为31，单体转化率为96.77%。超声处理被证明是一种有效的绿色方法，可增强聚酯化反应的初始动力学并提高聚合度。
