对自由活动大鼠运动行为的光遗传学调控
Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats.
作者信息
Xu Kedi, Zhang Jiacheng, Guo Songchao, Zheng Xiaoxiang
机构信息
Qiushi Academy for Advanced Studies (QAAS), Zhejiang University, Hangzhou, 310027, China.
Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education Ministry, Zhejiang University, Hangzhou, 310027, China.
出版信息
Methods Mol Biol. 2016;1408:195-206. doi: 10.1007/978-1-4939-3512-3_13.
The technology of optogenetics provides a new method to modulate neural activity with spatial specificity and millisecond-temporal scale. This nonelectrical modulation method also gives chance for simultaneous electrophysiological recording during stimulations. Here, we describe our locomotor activity modulation on free-behaving rats using optogenetic techniques. The target sites of the rat brain were dorsal periaqueductal gray (dPAG) and ventral tegmental area (VTA) for the modulation of defensive and reward behaviors, respectively.
光遗传学技术提供了一种以空间特异性和毫秒级时间尺度调节神经活动的新方法。这种非电调制方法也为在刺激过程中同时进行电生理记录提供了机会。在此，我们描述了我们使用光遗传学技术对自由活动大鼠的运动活动进行调制的情况。大鼠大脑的目标部位分别是中脑导水管周围灰质背侧（dPAG）和腹侧被盖区（VTA），用于调节防御行为和奖赏行为。
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