Fabian Peter, Pantzartzi Chrysoula N, Kozmikova Iryna, Kozmik Zbynek
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, Czech Republic.
Dev Genes Evol. 2016 Mar;226(2):99-107. doi: 10.1007/s00427-016-0533-8. Epub 2016 Mar 10.
The midbrain-hindbrain boundary (MHB) is one of the key organizing centers of the vertebrate central nervous system (CNS). Its patterning is governed by a well-described gene regulatory network (GRN) involving several transcription factors, namely, pax, gbx, en, and otx, together with signaling molecules of the Wnt and Fgf families. Here, we describe the onset of these markers in Oryzias latipes (medaka) early brain development in comparison to previously known zebrafish expression patterns. Moreover, we show for the first time that vox, a member of the vent gene family, is expressed in the developing neural tube similarly to CNS markers. Overexpression of vox leads to profound changes in the gene expression patterns of individual components of MHB-specific GRN, most notably of fgf8, a crucial organizer molecule of MHB. Our data suggest that genes from the vent family, in addition to their crucial role in body axis formation, may play a role in regionalization of vertebrate CNS.
中脑-后脑边界（MHB）是脊椎动物中枢神经系统（CNS）的关键组织中心之一。其模式形成受一个描述详尽的基因调控网络（GRN）支配，该网络涉及几种转录因子，即pax、gbx、en和otx，以及Wnt和Fgf家族的信号分子。在这里，我们描述了这些标记物在青鳉早期脑发育中的出现情况，并与先前已知的斑马鱼表达模式进行了比较。此外，我们首次表明，vent基因家族的成员vox在发育中的神经管中与中枢神经系统标记物的表达方式相似。vox的过表达导致MHB特异性GRN各个成分的基因表达模式发生深刻变化，最显著的是fgf8，它是MHB的关键组织分子。我们的数据表明，vent家族的基因除了在体轴形成中起关键作用外，可能在脊椎动物中枢神经系统的区域化中也发挥作用。
