Alarcón Gonzalo, Barraza Gabriela, Vera Andrea, Wozniak Aniela, García Patricia
Rev Chilena Infectol. 2016 Feb;33(1):26-9. doi: 10.4067/S0716-10182016000100004.
Trichomonas vaginalis, Mycoplasma hominis and Ureaplasma spp. are microorganisms responsible for genitourinary and pregnancy pathologies. Nucleic acid amplification methods have shown several advantages, but have not been widely studied for the detection of these microorganisms.
To implement a conventional polymerase chain reaction (PCR) for the detection of the microorganisms and to compare its results versus the methods currently used at our laboratory.
91 available samples were processed by PCR, culture (M. hominis y Ureaplasma spp.) and wet mount (T vaginalis). Results were compared and statistically analyzed by kappa agreement test.
85, 80 and 87 samples resulted in agreement for the detection of M. hominis, Ureaplasma spp. y T. vaginalis, respectively. For M. hominis and Ureaplasma spp., agreement was substantial, whereas for T. vaginalis it was moderate, however, for the latter, PCR detected more cases than wet mount.
We recommend the implementation of PCR for detection of T. vaginalis whereas culture kit is still a useful method for the other microorganisms.
阴道毛滴虫、人型支原体和解脲脲原体是引起泌尿生殖系统疾病和妊娠相关病症的微生物。核酸扩增方法已显示出多种优势，但尚未针对这些微生物的检测进行广泛研究。
实施一种用于检测这些微生物的常规聚合酶链反应（PCR），并将其结果与我们实验室目前使用的方法进行比较。
对91份可用样本进行PCR、培养（人型支原体和解脲脲原体）及湿片检查（阴道毛滴虫）处理。通过kappa一致性检验对结果进行比较和统计学分析。
分别有85、80和87份样本在人型支原体、解脲脲原体和阴道毛滴虫的检测上结果一致。对于人型支原体和解脲脲原体，一致性较高；而对于阴道毛滴虫，一致性为中等，不过，对于后者，PCR检测出的病例比湿片检查更多。
我们建议采用PCR检测阴道毛滴虫，而培养试剂盒仍是检测其他微生物的有用方法。
