一种新颖的分步预处理玉米秸秆的方法，通过碱脱乙酰化和液火热处理来提高酶解效率和能量利用率。
A novel stepwise pretreatment on corn stalk by alkali deacetylation and liquid hot water for enhancing enzymatic hydrolysis and energy utilization efficiency.
机构信息
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100039, China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
出版信息
Bioresour Technol. 2016 Jun;209:115-24. doi: 10.1016/j.biortech.2016.02.111. Epub 2016 Mar 2.
A novel stepwise pretreatment on corn stalk (CS) by alkali deacetylation combined with liquid hot water (LHW) was investigated to enhance enzymatic hydrolysis. After deacetylated treatment, strength of alkali deacetylation of CS was from 1.79% to 91.34% which was subsequently pretreated by LHW with severity from 3.27 to 4.27. It was found that higher strength of alkali deacetylation could reduce both the degradation of hemicellulose and inhibitors formation in liquid hot water pretreatment (LHWP). Enzymatic hydrolysis efficiency was confirmed to be affected by LHW pretreatment severity (PS) and strength of alkali treatment. This combined pretreatment of alkali deacetylation and LHW could not only increase glucose yield, but also enhance energy utilization efficiency. The maximum enzymatic hydrolysis of 87.55%±3.64 with the ratio of glucose yield to energy input at 50.39gglucosekJ(-1) was obtained when strength of alkali deacetylation at 84.96% with PS at 3.97 were used.
采用碱脱乙酰化结合液氨预处理对玉米秸秆（CS）进行了一种新的分步预处理，以提高酶水解效率。脱乙酰化处理后，CS 的碱脱乙酰化强度从 1.79%提高到 91.34%，随后用液氨预处理，严重度从 3.27 提高到 4.27。结果表明，较高的碱脱乙酰化强度可以降低液氨预处理（LHWP）中半纤维素的降解和抑制剂的形成。酶水解效率被证实受到液氨预处理严重度（PS）和碱处理强度的影响。碱脱乙酰化和液氨的联合预处理不仅可以提高葡萄糖产量，还可以提高能量利用效率。当碱脱乙酰化强度为 84.96%，PS 为 3.97 时，酶水解的最大葡萄糖得率为 87.55%±3.64，葡萄糖得率与能量投入比为 50.39gglucosekJ(-1)。
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