Naidek Karine P, Zuconelli Cristiane R, Cruz Otavio M, Ribeiro Ronny, Winnischofer Sheila M B, Winnischofer Herbert
a Universidade Federal do Paraná, Dep. Química, C.P. 19081, 81531-980, Curitiba, Paraná, Brazil.
b Universidade Federal do Paraná, Dep. Bioquímica e Biologia Molecular, C.P. 19046, 81531-980, Curitiba, Paraná, Brazil.
Biochem Cell Biol. 2016 Apr;94(2):205-11. doi: 10.1139/bcb-2015-0093. Epub 2016 Jan 18.
We synthesized 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP), characterized it by electrochemistry, spectroelectrochemistry, and electron paramagnetic resonance techniques, and evaluated its cytotoxicity to human cancer cell lines. The results revealed that HHTP has accessible higher-oxidation states, especially the tris-semiquinone monoradical. This species is stable and is formed after being stored for months. HHTP exhibited cytotoxic effects on 5 human cancer cell lines, including glioma and lung cancer cells. The cytotoxic effect was evaluated based on the decrease in cell viability, increases in the percentage of cells with fragmented DNA, and elevated numbers of annexin V-PI-positive cells after HHTP treatment.
我们合成了2,3,6,7,10,11-六羟基三亚苯（HHTP），通过电化学、光谱电化学和电子顺磁共振技术对其进行了表征，并评估了其对人癌细胞系的细胞毒性。结果表明，HHTP具有可及的较高氧化态，尤其是三半醌单自由基。该物种很稳定，在储存数月后形成。HHTP对5种人癌细胞系表现出细胞毒性作用，包括胶质瘤和肺癌细胞。基于HHTP处理后细胞活力的降低、DNA片段化细胞百分比的增加以及膜联蛋白V-PI阳性细胞数量的增加来评估细胞毒性作用。
