Johnson Michelle H, de Mejia Elvira Gonzalez
Division of Nutritional Sciences and ‡Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
J Agric Food Chem. 2016 Mar 30;64(12):2569-81. doi: 10.1021/acs.jafc.6b00239. Epub 2016 Mar 21.
Berries are a rich source of bioactive phenolic compounds that are able to bind and inhibit the enzyme dipeptidyl peptidase-IV (DPP-IV), a current target for type-2 diabetes therapy. The objectives were to determine the role of berry phenolic compounds to modulate incretin-cleaving DPP-IV and its substrate glucagon-like peptide-1 (GLP-1), insulin secretion from pancreatic β-cells, and genes and proteins involved in the insulin secretion pathway using cell culture. Anthocyanins (ANC) from 50% blueberry-50% blackberry (Blu-Bla) and 100% blackberry (Bla) fermented beverages at 50 μM cyanidin-3-glucoside equivalents increased (p < 0.05) glucose-stimulated insulin secretion from pancreatic β-cells (iNS-1E) both when applied directly and following simulated absorption through Caco-2 cells (by 233 and 100 μIU insulin/mL, respectively). ANC 50%Blu-Bla and ANC 100%Bla upregulated the gene for incretin hormone GLP-1 (fold-change 3.0 ± 1.4 and 2.0 ± 0.3, respectively) and genes in the insulin secretory pathway including insulin-like growth factor 1 receptor (iGF1R, 2.3 ± 0.6 and 1.6 ± 0.3, respectively), and increased (p < 0.05) the protein expression of insulin-like growth factor 2 (IGF-II), insulin-like growth factor binding proteins (IGFBP-2 and 3), and vascular endothelial growth factor (VEGF) in iNS-1E cells. Taken together, anthocyanins, predominantly delphinidin-3-arabinoside, from fermented berry beverages have the potential to modulate DPP-IV and its substrate GLP-1, to increase insulin secretion, and to upregulate expression of mRNA of insulin-receptor associated genes and proteins in pancreatic β-cells.
浆果富含生物活性酚类化合物，这些化合物能够结合并抑制二肽基肽酶-IV（DPP-IV），而DPP-IV是目前2型糖尿病治疗的靶点。本研究的目的是利用细胞培养来确定浆果酚类化合物在调节肠促胰岛素裂解DPP-IV及其底物胰高血糖素样肽-1（GLP-1）、胰腺β细胞胰岛素分泌以及胰岛素分泌途径中相关基因和蛋白质方面的作用。来自50%蓝莓-50%黑莓（蓝-黑）和100%黑莓（黑）发酵饮料中的花色苷（ANC），以50 μM矢车菊素-3-葡萄糖苷当量直接应用或经Caco-2细胞模拟吸收后，均增加了（p < 0.05）胰腺β细胞（iNS-1E）的葡萄糖刺激胰岛素分泌（分别为233和100 μIU胰岛素/mL）。50%蓝-黑ANC和100%黑ANC上调了肠促胰岛素激素GLP-1的基因（分别为3.0±1.4倍和2.0±0.3倍）以及胰岛素分泌途径中的基因，包括胰岛素样生长因子1受体（iGF1R，分别为2.3±0.6和1.6±0.3），并增加了（p < 0.05）iNS-1E细胞中胰岛素样生长因子2（IGF-II）、胰岛素样生长因子结合蛋白（IGFBP-2和3）以及血管内皮生长因子（VEGF）的蛋白表达。综上所述，来自发酵浆果饮料的花色苷，主要是飞燕草素-3-阿拉伯糖苷，有可能调节DPP-IV及其底物GLP-1，增加胰岛素分泌，并上调胰腺β细胞中胰岛素受体相关基因和蛋白质的mRNA表达。
