Yu Kyung Lee, Lee Sun Hee, Lee Eun Soo, You Ji Chang
National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seocho-gu Banpo-dong 505, Seoul 137-701, Republic of Korea.
National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seocho-gu Banpo-dong 505, Seoul 137-701, Republic of Korea.
Virology. 2016 May;492:204-12. doi: 10.1016/j.virol.2016.03.002. Epub 2016 Mar 21.
The HIV-1 nucleocapsid (NC) is an essential viral protein containing two highly conserved retroviral-type zinc finger (ZF) motifs, which functions in multiple stages of the HIV-1 life cycle. Although a number of functions for NC either in its mature form or as a domain of Gag have been revealed, little is known about the intracellular localization of NC and, moreover, its role in Gag protein trafficking. Here, we have investigated various forms of HIV-1 NC protein for its cellular localization and found that the NC has a strong nuclear and nucleolar localization activity. The linker region, composed of a stretch of basic amino acids between the two ZF motifs, was necessary and sufficient for the activity.
HIV-1核衣壳（NC）是一种重要的病毒蛋白，含有两个高度保守的逆转录病毒型锌指（ZF）基序，在HIV-1生命周期的多个阶段发挥作用。尽管已经揭示了NC成熟形式或作为Gag结构域的多种功能，但对于NC在细胞内的定位以及它在Gag蛋白运输中的作用却知之甚少。在这里，我们研究了HIV-1 NC蛋白的各种形式的细胞定位，发现NC具有很强的核和核仁定位活性。由两个ZF基序之间的一段碱性氨基酸组成的连接区对于该活性是必需且足够的。
