通过23,24-二降-4-胆甾烯-3-酮的倍半萜途径合成肾上腺皮质类固醇。

Biosynthesis of adrenal corticosteroids by the sesterterpene pathway via 23,24-dinor-4-cholen-3-one.

作者信息

Tait A D, Hodge L C, Abbs D

机构信息

Department of Obstetrics & Gynaecology, University of Cambridge, Addenbrooke's Hospital, England.

出版信息

J Steroid Biochem. 1989;34(1-6):563-5. doi: 10.1016/0022-4731(89)90146-5.

DOI:10.1016/0022-4731(89)90146-5

PMID:2696852

Abstract

Rat adrenal gland preparations were incubated with 23,24-dinor-5-cholen-3 beta-ol (Guneribol), a proposed intermediate in the sesterterpene pathway for steroid biosynthesis. Steroids were isolated, purified by thin layer and high-performance liquid chromatographies and crystallized to constant specific activity. These preparations converted the substrate to 23,24-dinor-4-cholen-3-one. Radioactive 23,24-dinor-4-cholen-3-one was synthesised and incubated with further tissue preparations and shown to be converted to corticosteroids. These findings suggest that 23,24-dinor-4-cholen-3-one is an intermediate on the sesterterpene pathway for steroidogenesis.

摘要

将大鼠肾上腺制剂与23,24-二降-5-胆甾烯-3β-醇（孕烯醇酮）一起孵育，孕烯醇酮是甾体生物合成的倍半萜途径中一种假定的中间体。分离甾体，通过薄层色谱法和高效液相色谱法进行纯化，并结晶至恒定的比活性。这些制剂将底物转化为23,24-二降-4-胆甾烯-3-酮。合成放射性23,24-二降-4-胆甾烯-3-酮，并与其他组织制剂一起孵育，结果表明其可转化为皮质类固醇。这些发现表明，23,24-二降-4-胆甾烯-3-酮是甾体生成的倍半萜途径中的一个中间体。