通过温和的碳酸钠预处理降低生物质顽固性。
Reducing biomass recalcitrance via mild sodium carbonate pretreatment.
作者信息
Mirmohamadsadeghi Safoora, Chen Zhu, Wan Caixia
机构信息
Department of Bioengineering, University of Missouri, Columbia, MO 65211, United States; Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Bioengineering, University of Missouri, Columbia, MO 65211, United States.
出版信息
Bioresour Technol. 2016 Jun;209:386-90. doi: 10.1016/j.biortech.2016.02.096. Epub 2016 Feb 27.
This study examined the effects of mild sodium carbonate (Na2CO3) pretreatment on enzymatic hydrolysis of different feedstocks (i.e., corn stover, Miscanthus, and switchgrass). The results showed that sodium carbonate pretreatment markedly enhanced the sugar yields of the tested biomass feedstocks. The pretreated corn stover, Miscanthus, and switchgrass gave the glucose yields of 95.1%, 62.3%, and 81.3%, respectively, after enzymatic hydrolysis. The above glucose yields of pretreated feedstocks were 2-4 times that of untreated ones. The pretreatment also enhanced the xylose yields, 4 times for corn stover and 20 times for both Miscanthus and switchgrass. Sodium carbonate pretreatment removed 40-59% lignin from the tested feedstocks while preserving most of cellulose (<5% cellulose loss). Corn stover appeared to be least resistant to breakdown by Na2CO3 and enzymatic hydrolysis. Our study indicated that mild sodium carbonate pretreatment was effective for reducing biomass recalcitrance and subsequently improving the digestibility of lignocellulosic biomass.
本研究考察了轻度碳酸钠（Na₂CO₃）预处理对不同原料（即玉米秸秆、芒草和柳枝稷）酶解的影响。结果表明，碳酸钠预处理显著提高了受试生物质原料的糖产率。经酶解后，预处理的玉米秸秆、芒草和柳枝稷的葡萄糖产率分别为95.1%、62.3%和81.3%。预处理原料的上述葡萄糖产率是未处理原料的2至4倍。预处理还提高了木糖产率，玉米秸秆提高了4倍，芒草和柳枝稷均提高了20倍。碳酸钠预处理从受试原料中去除了40%至59%的木质素，同时保留了大部分纤维素（纤维素损失<5%）。玉米秸秆似乎对Na₂CO₃分解和酶解的抗性最小。我们的研究表明，轻度碳酸钠预处理对于降低生物质顽固性并随后提高木质纤维素生物质的消化率是有效的。
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