Silva M A, Costa G M J, Lacerda S M S N, Brandão-Dias P F P, Kalapothakis E, Silva Júnior A F, Alvarenga E R, França L R
Laboratory of Cellular Biology (Dept. of Morphology), Federal University of Minas Gerais, MG 31270-901, Brazil.
Laboratory of Biotechnology and Molecular Markers (Dept. of General Biology), Federal University of Minas Gerais, MG 31270-901, Brazil.
Gen Comp Endocrinol. 2016 May 1;230-231:48-56. doi: 10.1016/j.ygcen.2016.03.012. Epub 2016 Mar 10.
Fish germ cell transplantation presents several important potential applications for aquaculture, including the preservation of germplasm from endangered fish species with high genetic and commercial values. Using this technique in studies developed in our laboratory with adult male Nile tilapias (Oreochromis niloticus), all the necessary procedures were successfully established, allowing the production of functional sperm and healthy progeny approximately 2months after allogeneic transplantation. In the present study, we evaluated the viability of the adult Nile tilapia testis to generate sperm after xenogeneic transplant of germ cells from sexually mature Jundia catfish (Rhamdia quelen) that belong to a different taxonomic order. Therefore, in order to investigate at different time-periods post-transplantation, the presence and development of donor PKH26 labeled catfish germ cells were followed in the tilapia seminiferous tubules. From 7 to 20days post-transplantation, only PKH26 labeled spermatogonia were observed, whereas spermatocytes at different stages of development were found at 70days. Germ cell transplantation success and progression of spermatogenesis were indicated by the presence of labeled PKH26 spermatids and sperm on days 90 and 120 post-transplantation, respectively. Confirming the presence of the catfish genetic material in the tilapia testis, all recipient tilapias evaluated (n=8) showed the genetic markers evaluated. Therefore, we demonstrated for the first time that the adult Nile tilapia testis offers the functional conditions for development of spermatogenesis with sperm production from a fish species belonging to a different order, which provides an important new venue for aquaculture advancement.
鱼类生殖细胞移植在水产养殖中具有几个重要的潜在应用，包括保存具有高遗传和商业价值的濒危鱼类的种质资源。在我们实验室对成年雄性尼罗罗非鱼（Oreochromis niloticus）开展的研究中使用了这项技术，成功建立了所有必要的程序，在同种异体移植后约2个月产生了功能性精子和健康后代。在本研究中，我们评估了成年尼罗罗非鱼睾丸在异种移植来自性成熟的军曹鱼（Rhamdia quelen）（属于不同分类目的鱼类）的生殖细胞后产生精子的可行性。因此，为了在移植后的不同时间段进行研究，在罗非鱼的生精小管中追踪供体PKH26标记的军曹鱼生殖细胞的存在和发育情况。移植后7至20天，仅观察到PKH26标记的精原细胞，而在70天时发现了不同发育阶段的精母细胞。移植后第90天和第120天分别出现标记的PKH26精子细胞和精子，表明生殖细胞移植成功和精子发生过程的进展。在评估的所有受体罗非鱼（n = 8）中均显示出所评估的遗传标记，从而证实了军曹鱼遗传物质在罗非鱼睾丸中的存在。因此，我们首次证明成年尼罗罗非鱼睾丸为来自不同目的鱼类产生精子的精子发生发育提供了功能条件，这为水产养殖的发展提供了一个重要的新途径。
