Kayo Daichi, Kanda Shinji, Okubo Kataaki
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Science, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.
Present address: Laboratory of Molecular Ethology, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980-8577, Japan.
Zoological Lett. 2022 Jul 25;8(1):10. doi: 10.1186/s40851-022-00195-1.
Generally, successful testis transplantation has been considered to require immune suppression in the recipient to avoid rejection of the transplanted tissue. In the present study, we demonstrate in medaka that allogeneic adult testicular tissue will engraft in adult recipients immediately after partial castration without the use of immunosuppressive drugs. The allografted testes are retained in the recipient's body for at least 3 months and are able to produce viable sperm that yield offspring after natural mating. Some recipients showed a high frequency (over 60%) of offspring derived from spermatozoa produced by the transplanted testicular tissue. Histological analyses showed that allografted testicular tissues included both germ cells and somatic cells that had become established within an immunocompetent recipient testis. The relative simplicity of this testis transplantation approach will benefit investigations of the basic processes of reproductive immunology and will improve the technique of gonadal tissue transplantation.
一般来说，成功的睾丸移植被认为需要对受体进行免疫抑制，以避免移植组织被排斥。在本研究中，我们在青鳉中证明，同种异体成年睾丸组织在部分阉割后可立即植入成年受体体内，而无需使用免疫抑制药物。异体移植的睾丸在受体体内保留至少3个月，并且能够产生可育精子，这些精子在自然交配后可产生后代。一些受体中，源自移植睾丸组织产生的精子的后代频率很高（超过60%）。组织学分析表明，异体移植的睾丸组织包含已在具有免疫活性的受体睾丸内定植的生殖细胞和体细胞。这种睾丸移植方法相对简单，将有助于生殖免疫学基本过程的研究，并将改进性腺组织移植技术。
