Miz-1的第13个C2H2锌指的溶液结构。
Solution structure of the 13th C2H2 Zinc Finger of Miz-1.
作者信息
Tremblay Cynthia, Bédard Mikaël, Bonin Marc-André, Lavigne Pierre
机构信息
Département de Biochimie, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Québec, Canada; Institut de Pharmacologie de Sherbrooke, Université de Sherbrooke, Sherbrooke, Canada; PROTEO; Regroupement Stratégique sur la Fonction, la Structure et l'Ingénierie des Protéines, Université Laval, Québec, Québec, Canada; GRASP; Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montréal, Québec, Canada.
Institut de Pharmacologie de Sherbrooke, Université de Sherbrooke, Sherbrooke, Canada.
出版信息
Biochem Biophys Res Commun. 2016 Apr 29;473(2):471-5. doi: 10.1016/j.bbrc.2016.03.034. Epub 2016 Mar 10.
Miz-1 is a BTB/POZ transcription factor that contains 13C2H2 Zinc Finger domains (ZF). Miz-1 transactivates and represses the transcription of a myriad of genes involved in many aspects of the biology of the cell. The detailed molecular interactions through which Miz-1 controls transcription, including its specific DNA binding via its ZF domains, remain to be understood and documented. In our effort to shed light into the structural biology of Miz-1, we have undertaken the determination of the structure of all its ZF and the characterization of their interactions with cognate DNA. The structure of ZF 1 to 10 have already been solved and characterized. Here, we present the structure of the synthetic Miz-1 ZF13 determined by 2D (1)H-(1)H NMR.
Miz-1是一种BTB/POZ转录因子，含有13个C2H2锌指结构域（ZF）。Miz-1可激活和抑制众多参与细胞生物学多个方面的基因的转录。Miz-1控制转录的详细分子相互作用，包括其通过ZF结构域与特定DNA的结合，仍有待了解和记录。为了深入了解Miz-1的结构生物学，我们已着手确定其所有ZF的结构，并表征它们与同源DNA的相互作用。ZF 1至10的结构已得到解析和表征。在此，我们展示通过二维（1）H-（1）H NMR测定的合成Miz-1 ZF13的结构。
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