Carter-Dawson L, Burroughs M
Sensory Sciences Center, Graduate School of Biomedical Sciences, University of Texas Health Science Center, Houston 77030.
Curr Eye Res. 1989 Dec;8(12):1331-4. doi: 10.3109/02713688909013914.
Light microscopic immunogold cytochemistry was used to examine the distribution of interphotoreceptor retinoid-binding protein (IRBP) in the interphotoreceptor matrix (IPM) surrounding rod and cone photoreceptors. Silver enhancement of retinas reacted with anti-IRBP antibodies using the two stage labeling procedure showed dense staining of the IPM around rod photoreceptor outer segments and the apical region of the RPE. However, the IPM around cone photoreceptors was lightly labeled for IRBP. This region of light labeling extended from the RPE to the distal one-fourth to one-third of the cone inner segment. Although most of the area surrounding cone outer segments was lightly labeled, a dense band of label was seen along the margins of cone outer segments. This study confirms that heterogeneity exists in the distribution of IRBP in the IPM and provides new evidence that IRBP is not homogeneously distributed around rod and cone photoreceptors.
采用光学显微镜免疫金细胞化学方法，研究了视网膜色素上皮（RPE）与光感受器间基质（IPM）中感光细胞间视黄醛结合蛋白（IRBP）的分布情况。用抗IRBP抗体，通过两步标记法对视网膜进行银增强处理，结果显示在视杆细胞外段周围的IPM和RPE顶端区域有密集染色。然而，视锥细胞周围的IPM中IRBP标记较浅。该浅标记区域从RPE延伸至视锥细胞内段的远端四分之一至三分之一处。虽然视锥细胞外段周围的大部分区域标记较浅，但在视锥细胞外段边缘可见一条密集的标记带。本研究证实，IPM中IRBP的分布存在异质性，并提供了新的证据，表明IRBP在视杆和视锥光感受器周围并非均匀分布。
