Kim Sang Hoon, Pajarillo Edward Alain B, Balolong Marilen P, Lee Ji Yoon, Kang Dae-Kyung
Department of Animal Resources Science, Dankook, University, Cheonan 31116, Republic of Korea.
National Instrumentation Center for Environmental Management, Seoul National University, Seoul 08826, Republic of Korea.
J Microbiol Biotechnol. 2016 Jun 28;26(6):1124-31. doi: 10.4014/jmb.1512.12075.
In this study, the global proteome of the IPEC-J2 cell line was evaluated using ultra-high performance liquid chromatography coupled to a quadrupole Q Exactive™ Orbitrap mass spectrometer. Proteins were isolated from highly confluent IPEC-J2 cells in biological replicates and analyzed by label-free mass spectrometry prior to matching against a porcine genomic dataset. The results identified 1,517 proteins, accounting for 7.35% of all genes in the porcine genome. The highly abundant proteins detected, such as actin, annexin A2, and AHNAK nucleoprotein, are involved in structural integrity, signaling mechanisms, and cellular homeostasis. The high abundance of heat shock proteins indicated their significance in cellular defenses, barrier function, and gut homeostasis. Pathway analysis and annotation using the Kyoto Encyclopedia of Genes and Genomes database resulted in a putative protein network map of the regulation of immunological responses and structural integrity in the cell line. The comprehensive proteome analysis of IPEC-J2 cells provides fundamental insights into overall protein expression and pathway dynamics that might be useful in cell adhesion studies and immunological applications.
在本研究中，使用超高效液相色谱与四极杆Q Exactive™ 轨道阱质谱仪联用，对IPEC-J2细胞系的全蛋白质组进行了评估。从高度汇合的IPEC-J2细胞中分离蛋白质，进行生物学重复，并通过无标记质谱分析，然后与猪基因组数据集进行比对。结果鉴定出1517种蛋白质，占猪基因组所有基因的7.35%。检测到的高丰度蛋白质，如肌动蛋白、膜联蛋白A2和AHNAK核蛋白，参与结构完整性、信号传导机制和细胞内稳态。热休克蛋白的高丰度表明它们在细胞防御、屏障功能和肠道内稳态中的重要性。使用京都基因与基因组百科全书数据库进行通路分析和注释，得到了该细胞系免疫反应调节和结构完整性的推测蛋白质网络图。对IPEC-J2细胞的全面蛋白质组分析为整体蛋白质表达和通路动态提供了基本见解，这可能在细胞黏附研究和免疫学应用中有用。
