Bai Xuejia, Huang Wenqiu, Zhang Chenguang, Niu Jing, Ding Wei
Department of Medical Genetics, Capital Medical University, Beijing, P.R. China.
Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, P.R. China.
Anticancer Res. 2016 Mar;36(3):1203-10.
One of the basic leucine zipper transcription factors, BATF2, has been found to suppress cancer growth and migration. However, little is known about the genes downstream of BATF2.
HeLa cells were stably transfected with BATF2, then chromatin immunoprecipitation-sequencing was employed to identify the DNA motifs responsive to BATF2.
Comprehensive bioinformatics analyses indicated that the most significant motif discovered as TTCCATT[CT]GATTCCATTC[AG]AT was primarily distributed among the chromosome centromere regions and mostly within human type II satellite DNA. Such motifs were able to prime the transcription of type II satellite DNA in a directional and asymmetrical manner. Consistently, satellite II transcription was up-regulated in BATF2-overexpressing cells.
The present study provides insight into understanding the role of BATF2 in tumours and the importance of satellite DNA in the maintenance of genomic stability.
已发现基本亮氨酸拉链转录因子之一的BATF2可抑制癌症生长和迁移。然而，关于BATF2下游的基因知之甚少。
用BATF2稳定转染HeLa细胞，然后采用染色质免疫沉淀测序法来鉴定对BATF2有反应的DNA基序。
全面的生物信息学分析表明，发现的最显著基序为TTCCATT[CT]GATTCCATTC[AG]AT，主要分布在染色体着丝粒区域，且大多位于人类II型卫星DNA内。此类基序能够以定向和不对称的方式启动II型卫星DNA的转录。同样，在BATF2过表达的细胞中卫星II转录上调。
本研究为理解BATF2在肿瘤中的作用以及卫星DNA在维持基因组稳定性中的重要性提供了见解。
