Vojta Lea, Fulgosi Hrvoje
Laboratory for Molecular Plant Biology and Biotechnology, Division of Molecular Biology, Institute Ruđer Bošković, 10 000 Zagreb, Croatia.
Data Brief. 2016 Feb 26;7:393-6. doi: 10.1016/j.dib.2016.02.044. eCollection 2016 Jun.
In photosynthesis, the flavoenzyme ferredoxin:NADP(+) oxidoreductase (FNR) catalyses the final electron transfer from ferredoxin to NADP(+), which is considered as the main pathway of high-energy electron partitioning in chloroplasts (DOI: 10.1111/j.1365-313X.2009.03999.x[1], DOI: 10.1038/srep10085[2]). Different detergents and pH treatments of photosynthetic membranes isolated from the Arabidopsis wild-type (WT) and the loss-of-function mutants of the thylakoid rhodanase-like protein TROL (trol), pre-acclimated to either dark, growth-light, or high-light conditions, were used to probe the strength of FNR-membrane associations. Detergents β-DM (decyl-β-D-maltopyranoside) or β-DDM (n-dodecyl-β-D-maltopyranoside) were used to test the stability of FNR binding to the thylakoid membranes, and to assess different membrane domains containing FNR. Further, the extraction conditions mimicked pH status of chloroplast stroma during changing light regimes. Plants without TROL are incapable of the dynamic FNR recruitment to the photosynthetic membranes.
在光合作用中，黄素酶铁氧还蛋白：NADP(+)氧化还原酶（FNR）催化从铁氧还蛋白到NADP(+)的最终电子转移，这被认为是叶绿体中高能电子分配的主要途径（DOI: 10.1111/j.1365-313X.2009.03999.x[1]，DOI: 10.1038/srep10085[2]）。对从拟南芥野生型（WT）以及类硫氰酸酶蛋白TROL（trol）功能缺失突变体中分离出的光合膜进行不同去污剂处理和pH处理，这些光合膜预先适应了黑暗、生长光或高光条件，以此来探究FNR与膜结合的强度。使用去污剂β-DM（癸基-β-D-麦芽糖苷）或β-DDM（正十二烷基-β-D-麦芽糖苷）来测试FNR与类囊体膜结合的稳定性，并评估含有FNR的不同膜结构域。此外，提取条件模拟了光照条件变化期间叶绿体基质的pH状态。没有TROL的植物无法将FNR动态招募到光合膜上。
