在单个肌管中利用荧光寿命成像显微镜进行直接细胞器温度测量。

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes.

作者信息

Itoh Hideki, Arai Satoshi, Sudhaharan Thankiah, Lee Sung-Chan, Chang Young-Tae, Ishiwata Shin'ichi, Suzuki Madoka, Lane E Birgitte

机构信息

Department of Pure and Applied Physics, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan and Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore.

Organization for University Research Initiatives, Waseda University, Tokyo 162-0041, Japan.

出版信息

Chem Commun (Camb). 2016 Mar 25;52(24):4458-61. doi: 10.1039/c5cc09943a.

DOI:10.1039/c5cc09943a

PMID:26977478

Abstract

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca(2+) burst.

摘要

我们描述了使用一种靶向内质网的小分子染料和胞质mCherry进行细胞器温度测量的方法，其荧光寿命随温度升高而降低，可通过荧光寿命成像显微镜进行监测。结果表明，单个肌管中的产热高度局部化，并与Ca(2+)爆发相关联。